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Title: S-Layer Nanosheet Binding of Zn and Gd

This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
Authors:
; ;
Publication Date:
Report Number(s):
951
DOE Contract Number:
FY15 AOP 2.5.1.4
Product Type:
Dataset
Research Org(s):
DOE Geothermal Data Repository; Lawrence Berkeley National Laboratory
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Geothermal Technologies Program (EE-2C)
Subject:
15 Geothermal Energy; geothermal; energy; mineral recovery; synthetic biology; S-layer; metal-binding domain; nanosheet; brine study; binding; chemistry; Gadolinium; zinc; gd3+; zn2+; ion
OSTI Identifier:
1372392

Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States: N. p., Web. doi:10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, & Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States. doi:10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. 2016. "S-Layer Nanosheet Binding of Zn and Gd". United States. doi:10.15121/1372392. https://www.osti.gov/servlets/purl/1372392.
@misc{osti_1372392,
title = {S-Layer Nanosheet Binding of Zn and Gd},
author = {Ajo-Franklin, Caroline and Charrier, Marimikel and Yang, Li},
abstractNote = {This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.},
doi = {10.15121/1372392},
year = {2016},
month = {4} }
  1. The Geothermal Data Repository (GDR) is the submission point for all data collected from researchers funded by the U.S. Department of Energy's Geothermal Technologies Office (DOE GTO). The DOE GTO is providing access to its geothermal project information through the GDR. The GDR is powered by OpenEI, an energy information portal sponsored by the U.S. Department of Energy and developed by the National Renewable Energy Laboratory (NREL).
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