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Title: X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex (CXIDB ID 32)

Authors:
Publication Date:
Report Number(s):
CXIDB ID 32
DOE Contract Number:
AC02-05CH11231
Product Type:
Dataset
Research Org(s):
Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Van Andel Research Institute; Center for Free Electron Laser Science; Arizona State Univ., Tempe, AZ (United States); University of Southern California; SLAC National Accelerator Laboratory; Paul Scherrer Institute; Trinity College; Shanghai Tech University; Chinese Academy of Sciences
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); Van Andel Research Institute; Center for Free Electron Laser Science; Arizona State University; University of Southern California; SLAC National Accelerator Laboratory; Paul Scherrer Institute; Trinity College; Shanghai Tech University; Chinese Academy of Sciences
Resource Relation:
Related Information: X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex, X. Edward Zhou et al. Scientific Data, 2015, http://dx.doi.org/TODO
Subject:
XFEL; X-ray Free-electorn Lasers; Serial Femtosecond Crystallography; Rhodopsin-arrestin complex; CXI; LCLS
OSTI Identifier:
1241101
  1. The Coherent X-ray Imaging Data Bank (CXIDB) is a new database which offers scientists from all over the world a unique opportunity to access data from Coherent X-ray Imaging (CXI) experiments. The main goal of the Coherent X-ray Imaging Data Bank is to create an open repository for CXI experimental data. CXIDB is dedicated to further the goal of making data from Coherent X-ray Imaging (CXI) experiments available to all, as well as archiving it. The website also serves as the reference for the CXI file format, in which most of the experimental data on the database is stored in.
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  1. Facilitating the very short and intense pulses from an X-ray laser for the purpose of imaging small bioparticles carries the potential for structure determination at atomic resolution without the need for crystallization. In this study, we explore experimental strategies for this idea based on datamore » collected at the Linac Coherent Light Source from 40 nm virus particles injected into a hard X-ray beam. « less
  2. These 2000 single-shot diffraction patterns include were either background-scattering only or hits (background-scattering plus diffraction signal from sub-micron ellipsoidal particles at random, undetermined orientations). Candidate hits were identified by eye, and the remainder were presumed as background. 54 usable, background-subtracted hits in this set (proceduremore » in referenced article) were used to reconstruct the 3D diffraction intensities of the average ellipsoidal particle. « less
  3. Please check the README file inside the metadata directory for more information about the dataset.
  4. These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 1 are themore » pattern and configuration files for the pattern showed in Figure 2a in the paper. « less