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Title: Imaging single cells in a beam of live cyanobacteria with an X-ray laser (CXIDB ID 27)

Diffraction pattern of a micron-sized S. elongatus cell at 1,100 eV photon energy (1.13 nm wavelength) with ~10^11 photons per square micron on the sample in ~70 fs. The signal to noise ratio at 4 nm resolution is 3.7 with 0.24 photons per Nyquist pixel. The cell was alive at the time of the exposure. The central region of the pattern (dark red) is saturated and this prevented reliable image reconstruction.
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Research Org(s):
Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Uppsala University
Sponsoring Org:
Uppsala University
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Related Information: Imaging single cells in a beam of live cyanobacteria with an X-ray laser, Gijs van der Schot et al. Nature Communications, 2015,
Synechococcus elongatus; XFEL; AMO; Single Particle X-ray Diffraction Imaging; X-ray Free-electorn Lasers; LCLS
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  1. The Coherent X-ray Imaging Data Bank (CXIDB) is a new database which offers scientists from all over the world a unique opportunity to access data from Coherent X-ray Imaging (CXI) experiments. The main goal of the Coherent X-ray Imaging Data Bank is to create an open repository for CXI experimental data. CXIDB is dedicated to further the goal of making data from Coherent X-ray Imaging (CXI) experiments available to all, as well as archiving it. The website also serves as the reference for the CXI file format, in which most of the experimental data on the database is stored in.
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