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Title: Single mimivirus particles intercepted and imaged with an X-ray laser (CXIDB ID 2)

These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 2 are the pattern and configuration files for the pattern showed in Figure 2b in the paper.
Authors:
;
Publication Date:
Report Number(s):
CXIDB ID 2
DOE Contract Number:
AC02-05CH11231
Product Type:
Dataset
Research Org(s):
Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Uppsala University
Collaborations:
CFEL DESY; University of Hamburg; CNRS Aix-Marseille Universitat; SLAC National Accelerator Laboratory; Swedish University of Agricultural Sciences; Arizona State University; Lawrence Berkeley National Laboratory (LBNL); Max-Planck-Institut fur medizinische Forschung; Max-Planck-Institut fur Kernphysik; Max-Planck-Institut Halbleiterlabor; Max-Planck-Institut fur extraterrestrische Physik; PNSensor GmbH; Universitat Siegen; Forschungszentrum Julich; TU Berlin
Sponsoring Org:
USDOE Office of Science (SC), Advanced Scientific Computing Research (ASCR) (SC-21)
Resource Relation:
Related Information: Single mimivirus particles intercepted and imaged with an X-ray laser, M. Marvin Seibert, Tomas Ekeberg, Filipe R. N. C. Maia et al. Nature, 2011, http://dx.doi.org/10.1038/nature09748
Subject:
Mimivirus; XFEL; AMO; Single Particle X-ray Diffraction Imaging; X-ray Free-electorn Lasers; LCLS
OSTI Identifier:
1096904
  1. The Coherent X-ray Imaging Data Bank (CXIDB) is a new database which offers scientists from all over the world a unique opportunity to access data from Coherent X-ray Imaging (CXI) experiments. The main goal of the Coherent X-ray Imaging Data Bank is to create an open repository for CXI experimental data. CXIDB is dedicated to further the goal of making data from Coherent X-ray Imaging (CXI) experiments available to all, as well as archiving it. The website also serves as the reference for the CXI file format, in which most of the experimental data on the database is stored in.
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  1. These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 1 are themore » pattern and configuration files for the pattern showed in Figure 2a in the paper. « less
  2. Preprocessed detector images that were used for the paper "High-throughput imaging of heterogeneous cell organelles with an X-ray laser". The CXI file contains the entire recorded data - including both hits and blanks. It also includes down-sampled images and LCLS machine parameters. Additionally, the Cheetahmore » configuration file is attached that was used to create the pre-processed data. « less
  3. This dataset contains the diffraction patterns that were used for the first three-dimensional reconstruction of a virus using FEL data. The sample was the giant mimivirus particle, which is one of the largest known viruses with a diameter of 450 nm. The dataset consists ofmore » the 198 diffraction patterns that were used in the analysis. « less
  4. These 2000 single-shot diffraction patterns include were either background-scattering only or hits (background-scattering plus diffraction signal from sub-micron ellipsoidal particles at random, undetermined orientations). Candidate hits were identified by eye, and the remainder were presumed as background. 54 usable, background-subtracted hits in this set (proceduremore » in referenced article) were used to reconstruct the 3D diffraction intensities of the average ellipsoidal particle. « less
  5. This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noisemore » levels. The cells come from various stages of the cell cycle, and were imaged in random orientations. « less