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Title: Subunit Compositions of the RNA-Silencing Enzymes Pol IV and Pol V Reveal Their Origins as Specialized Forms of RNA Polymerase II

Abstract

In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and gene silencing. We show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the 12 subunits of Pol II. Four subunits of Pol IV are distinct from their Pol II paralogs, six subunits of Pol V are distinct from their Pol II paralogs, and four subunits differ between Pol IV and Pol V. Importantly, the subunit differences occur in key positions relative to the template entry and RNA exit paths. Our findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
993360
Report Number(s):
PNNL-SA-75446
KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Molecular Cell, 33(2):192-203
Additional Journal Information:
Journal Name: Molecular Cell, 33(2):192-203
Country of Publication:
United States
Language:
English

Citation Formats

Ream, Thomas S, Haag, J R, Wierzbicki, A T, Nicora, Carrie D, Norbeck, Angela D, Zhu, Jian-Kang, Hagen, Gretchen, Guilfoyle, Thomas J, Pasa-Tolic, Ljiljana, and Pikaard, Craig S. Subunit Compositions of the RNA-Silencing Enzymes Pol IV and Pol V Reveal Their Origins as Specialized Forms of RNA Polymerase II. United States: N. p., 2009. Web. doi:10.1016/j.molcel.2008.12.015.
Ream, Thomas S, Haag, J R, Wierzbicki, A T, Nicora, Carrie D, Norbeck, Angela D, Zhu, Jian-Kang, Hagen, Gretchen, Guilfoyle, Thomas J, Pasa-Tolic, Ljiljana, & Pikaard, Craig S. Subunit Compositions of the RNA-Silencing Enzymes Pol IV and Pol V Reveal Their Origins as Specialized Forms of RNA Polymerase II. United States. https://doi.org/10.1016/j.molcel.2008.12.015
Ream, Thomas S, Haag, J R, Wierzbicki, A T, Nicora, Carrie D, Norbeck, Angela D, Zhu, Jian-Kang, Hagen, Gretchen, Guilfoyle, Thomas J, Pasa-Tolic, Ljiljana, and Pikaard, Craig S. 2009. "Subunit Compositions of the RNA-Silencing Enzymes Pol IV and Pol V Reveal Their Origins as Specialized Forms of RNA Polymerase II". United States. https://doi.org/10.1016/j.molcel.2008.12.015.
@article{osti_993360,
title = {Subunit Compositions of the RNA-Silencing Enzymes Pol IV and Pol V Reveal Their Origins as Specialized Forms of RNA Polymerase II},
author = {Ream, Thomas S and Haag, J R and Wierzbicki, A T and Nicora, Carrie D and Norbeck, Angela D and Zhu, Jian-Kang and Hagen, Gretchen and Guilfoyle, Thomas J and Pasa-Tolic, Ljiljana and Pikaard, Craig S},
abstractNote = {In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and gene silencing. We show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the 12 subunits of Pol II. Four subunits of Pol IV are distinct from their Pol II paralogs, six subunits of Pol V are distinct from their Pol II paralogs, and four subunits differ between Pol IV and Pol V. Importantly, the subunit differences occur in key positions relative to the template entry and RNA exit paths. Our findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense.},
doi = {10.1016/j.molcel.2008.12.015},
url = {https://www.osti.gov/biblio/993360}, journal = {Molecular Cell, 33(2):192-203},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 30 00:00:00 EST 2009},
month = {Fri Jan 30 00:00:00 EST 2009}
}