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Title: Development of Spectral Pattern Matching Approaches to Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry for Bacterial Identification

Abstract

The concept of rapid microorganism identification using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) dates back to the mid-1990’s. Prior to 1998, researchers relied on visual inspection in an effort to demonstrate feasibility of MALDI-MS for bacterial identification (Holland, Wilkes et al. 1996), (Krishnamurthy and Ross 1996), (Claydon, Davey et al. 1996). In general, researchers in these early studies visually compared the biomarker intensity profiles between different organisms and between replicates of the same organism to show that MALDI signatures are unique and reproducible. Manual tabulation and comparison of potential biomarker mass values observed for different organisms was used by numerous researchers to qualitatively characterize microorganisms using MALDI-MS spectra (e.g. (Lynn, Chung et al. 1999), (Winkler, Uher et al. 1999), (Ryzhov, Hathout et al. 2000), (Nilsson 1999)).

Authors:
;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
990604
Report Number(s):
PNNL-SA-43832
TRN: US201020%%306
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Book
Resource Relation:
Related Information: Identification of Microorganisms by Mass Spectrometry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; BACTERIA; IDENTIFICATION SYSTEMS; TIME-OF-FLIGHT MASS SPECTROMETERS; MASS SPECTROSCOPY; MASS SPECTRA; PATTERN RECOGNITION; DESORPTION; LASER RADIATION; IONIZATION

Citation Formats

Jarman, Kristin H., and Wahl, Karen L.. Development of Spectral Pattern Matching Approaches to Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry for Bacterial Identification. United States: N. p., 2005. Web.
Jarman, Kristin H., & Wahl, Karen L.. Development of Spectral Pattern Matching Approaches to Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry for Bacterial Identification. United States.
Jarman, Kristin H., and Wahl, Karen L.. Thu . "Development of Spectral Pattern Matching Approaches to Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry for Bacterial Identification". United States. doi:.
@article{osti_990604,
title = {Development of Spectral Pattern Matching Approaches to Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry for Bacterial Identification},
author = {Jarman, Kristin H. and Wahl, Karen L.},
abstractNote = {The concept of rapid microorganism identification using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) dates back to the mid-1990’s. Prior to 1998, researchers relied on visual inspection in an effort to demonstrate feasibility of MALDI-MS for bacterial identification (Holland, Wilkes et al. 1996), (Krishnamurthy and Ross 1996), (Claydon, Davey et al. 1996). In general, researchers in these early studies visually compared the biomarker intensity profiles between different organisms and between replicates of the same organism to show that MALDI signatures are unique and reproducible. Manual tabulation and comparison of potential biomarker mass values observed for different organisms was used by numerous researchers to qualitatively characterize microorganisms using MALDI-MS spectra (e.g. (Lynn, Chung et al. 1999), (Winkler, Uher et al. 1999), (Ryzhov, Hathout et al. 2000), (Nilsson 1999)).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Dec 01 00:00:00 EST 2005},
month = {Thu Dec 01 00:00:00 EST 2005}
}

Book:
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