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Title: Photothermal spectroscopy of Bacillus anthracis and Bacillus cereus with microcantilevers

Abstract

Microcalorimetric optical and infrared spectroscopy is a method of determining the spectral absorption of small quantities of materials over a wide range of incident wavelengths. In this paper, the first spectroscopic results for microcantilevers coated with Bacillus anthracis (BA) are presented. These results, for B. anthracis from 2.5 to 14.5 {micro}m, are compared with results from microcantilevers coated with Bacillus cereus (BC) and standard spectroscopic absorption data. The results demonstrate strong correlation between the deflection measurements and the reference spectroscopic absorption peaks. An advantage of this microcantilever-based method over traditional spectroscopy is that much smaller amounts of material (nanogram quantities) can be detected in comparison with the milligram amounts needed for standard methods. Another advantage is that the complete system can be relatively small without sacrificing spectral resolution.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
989539
DOE Contract Number:
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Sensors and Actuators B: Chemical; Journal Volume: 114; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ABSORPTION; BACILLUS; BACILLUS CEREUS; RESOLUTION; SPECTROSCOPY; WAVELENGTHS; microcantilever; sensor; photothermal

Citation Formats

Wig, Andrew G, Arakawa, Edward T, Passian, Ali, Ferrell, Thomas L, and Thundat, Thomas George. Photothermal spectroscopy of Bacillus anthracis and Bacillus cereus with microcantilevers. United States: N. p., 2006. Web. doi:10.1016/j.snb.2005.04.029.
Wig, Andrew G, Arakawa, Edward T, Passian, Ali, Ferrell, Thomas L, & Thundat, Thomas George. Photothermal spectroscopy of Bacillus anthracis and Bacillus cereus with microcantilevers. United States. doi:10.1016/j.snb.2005.04.029.
Wig, Andrew G, Arakawa, Edward T, Passian, Ali, Ferrell, Thomas L, and Thundat, Thomas George. Wed . "Photothermal spectroscopy of Bacillus anthracis and Bacillus cereus with microcantilevers". United States. doi:10.1016/j.snb.2005.04.029.
@article{osti_989539,
title = {Photothermal spectroscopy of Bacillus anthracis and Bacillus cereus with microcantilevers},
author = {Wig, Andrew G and Arakawa, Edward T and Passian, Ali and Ferrell, Thomas L and Thundat, Thomas George},
abstractNote = {Microcalorimetric optical and infrared spectroscopy is a method of determining the spectral absorption of small quantities of materials over a wide range of incident wavelengths. In this paper, the first spectroscopic results for microcantilevers coated with Bacillus anthracis (BA) are presented. These results, for B. anthracis from 2.5 to 14.5 {micro}m, are compared with results from microcantilevers coated with Bacillus cereus (BC) and standard spectroscopic absorption data. The results demonstrate strong correlation between the deflection measurements and the reference spectroscopic absorption peaks. An advantage of this microcantilever-based method over traditional spectroscopy is that much smaller amounts of material (nanogram quantities) can be detected in comparison with the milligram amounts needed for standard methods. Another advantage is that the complete system can be relatively small without sacrificing spectral resolution.},
doi = {10.1016/j.snb.2005.04.029},
journal = {Sensors and Actuators B: Chemical},
number = 1,
volume = 114,
place = {United States},
year = {Wed Mar 01 00:00:00 EST 2006},
month = {Wed Mar 01 00:00:00 EST 2006}
}