An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging

Journal Article · Wed Mar 01 00:00:00 EST 2006 · Medical Engineering & Physics

DOI:https://doi.org/10.1016/j.medengphy.2005.04.022· OSTI ID:989533

Martin, Matthew E ^[1]; Wabuyele, Musundi B ^[1]; Overholt, Bergein F ^[1]; Kennel, Steve J ^[1]; Cunningham, Glenn ^[1]; Vo Dinh, Tuan ^[1]

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1. ORNL

An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTF, and two cameras coupled with optics and electronics. Initial results show that the system can delineate normal and malignant mouse tissues real-time. The analysis shows that malignant tissues consistently exhibit less fluorescent intensity in the wavelength band from 440 to 540nm with a peak intensity of around 490nm. The analysis also shows key spectroscopic differences between normal and malignant tissues. Further, these results are compared to real-time spectroscopic data and show good correlation.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 989533
• Journal Information: Medical Engineering & Physics, Vol. 28, Issue 2; ISSN 1350-4533
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ACOUSTICS
CAMERAS
FILTERS
FLUORESCENCE
REFLECTION
MICE
ANIMAL TISSUES
LASERS
REAL TIME SYSTEMS
NEOPLASMS
hyperspectral fluorescence spectroscopy
acousto-optic tunable filters
medical imaging
biomedical optics
laser-induced fluorescence