Lignin-blocking treatment of biomass and uses thereof

Yang, Bin; Wyman, Charles E

Lignin-blocking treatment of biomass and uses thereof

Patent · 20 October 2009

OSTI ID:989010

Yang, Bin ^[1]; Wyman, Charles E ^[2]

Hanover, NH
Norwich, VT

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion. Cellulase efficiencies are improved by the protein or polypeptide treatment. The treatment may be used in combination with steam explosion and acid prehydrolysis techniques. Hydrolysis yields from lignin containing biomass are enhanced 5-20%, and enzyme utilization is increased from 10% to 50%. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.

View Patent

Research Organization:: Dartmouth College

Sponsoring Organization:: USDOE Office of Solar Thermal, Biomass Power, and Hydrogen Technologies (EE-13)

DOE Contract Number:: FC36-00GO10589; FC36-01GO11075;

Assignee:: The Trustees of Dartmouth College (Hanover, NH)

Patent Number(s):: 7,604,967

Application Number:: 10/391,740

OSTI ID:: 989010

Country of Publication:: United States

Language:: English

References (23)

Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates Yang, Bin; Boussaid, Abdel; Mansfield, Shawn D. Biotechnology and Bioengineering, Vol. 77, Issue 6 https://doi.org/10.1002/bit.10159	journal	January 2002
Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification Gould, J. Michael Biotechnology and Bioengineering, Vol. 26, Issue 1 https://doi.org/10.1002/bit.260260110	journal	January 1984
Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding Ghose, T. K.; Roychoudhury, P. K.; Ghosh, P. Biotechnology and Bioengineering, Vol. 26, Issue 4 https://doi.org/10.1002/bit.260260414	journal	April 1984
Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel Biotechnology Letters, Vol. 14, Issue 5 https://doi.org/10.1007/BF01021255	journal	May 1992
Thermotolerant yeast for simultaneous saccharification and fermentation of cellulose to ethanol Spindler, Diane D.; Wyman, Charles E.; Mohagheghi, Ali Applied Biochemistry and Biotechnology, Vol. 17, Issue 1-3, p. 279-293 https://doi.org/10.1007/BF02779163	journal	April 1988
Evaluation of pretreated herbaceous crops for the simultaneous saccharification and fermentation process Spindler, Diana; Wyman, Charles; Grohmann, Karel Applied Biochemistry and Biotechnology, Vol. 24-25, Issue 1 https://doi.org/10.1007/BF02920252	journal	March 1990
Dilute-acid pretreatment of two short-rotation herbaceous crops: Scientific Note Torget, R.; Himmel, M.; Grohmann, K. Applied Biochemistry and Biotechnology, Vol. 34-35, Issue 1 https://doi.org/10.1007/BF02920538	journal	March 1992
The simultaneous saccharification and fermentation of pretreated woody crops to ethanol Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel Applied Biochemistry and Biotechnology, Vol. 28-29, Issue 1 https://doi.org/10.1007/BF02922648	journal	March 1991
Simultaneous saccharification and fermentation of lignocellulose: Process evaluation Wright, John D.; Wyman, Charles E.; Grohmann, Karel Applied Biochemistry and Biotechnology, Vol. 18, Issue 1 https://doi.org/10.1007/BF02930818	journal	August 1988
Simultaneous saccharification and fermentation of pretreated wheat straw to ethanol with selected yeast strains and β-glucosidase supplementation Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel Applied Biochemistry and Biotechnology, Vol. 20-21, Issue 1 https://doi.org/10.1007/BF02936507	journal	January 1989
High solids simultaneous saccharification and fermentation of pretreated wheat straw to ethanol Mohagheghi, A.; Tucker, M.; Grohmann, K. Applied Biochemistry and Biotechnology, Vol. 33, Issue 2 https://doi.org/10.1007/BF02950778	journal	May 1992
Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan Chernoglazov, Vladimir M.; Ermolova, Olga V.; Klyosov, Anatole A. Enzyme and Microbial Technology, Vol. 10, Issue 8, p. 503-507 https://doi.org/10.1016/0141-0229(88)90029-4	journal	August 1988
Ethanol from lignocellulosic biomass: Technology, economics, and opportunities Wyman, Charles E. Bioresource Technology, Vol. 50, Issue 1, p. 3-15 https://doi.org/10.1016/0960-8524(94)90214-3	journal	January 1994
Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol Wyman, Charles E.; Spindler, Diane D.; Grohmann, Karel Biomass and Bioenergy, Vol. 3, Issue 5 https://doi.org/10.1016/0961-9534(92)90001-7	journal	January 1992
Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose Eriksson, Torny; Börjesson, Johan; Tjerneld, Folke Enzyme and Microbial Technology, Vol. 31, Issue 3 https://doi.org/10.1016/S0141-0229(02)00134-5	journal	August 2002
Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives Sewalt, V. J. H.; Glasser, W. G.; Beauchemin, K. A. Journal of Agricultural and Food Chemistry, Vol. 45, Issue 5 https://doi.org/10.1021/jf9608074	journal	May 1997
Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments [and Discussion] Overend, R. P.; Chornet, E.; Gascoigne, J. A. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 321, Issue 1561 https://doi.org/10.1098/rsta.1987.0029	journal	April 1987
Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges Wyman, Charles E. Annual Review of Energy and the Environment, Vol. 24, Issue 1, p. 189-226 https://doi.org/10.1146/annurev.energy.24.1.189	journal	November 1999
Optimization of Steam Explosion to Enhance Hemicellulose Recovery and Enzymatic Hydrolysis of Cellulose in Softwoods Wu, Michael M.; Chang, Kevin; Gregg, David J. Applied Biochemistry and Biotechnology, Vol. 77, Issue 1-3 https://doi.org/10.1385/ABAB:77:1-3:47	journal	January 1999
Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology: Selected Key Events Along the Way Wyman, Charles E. Applied Biochemistry and Biotechnology, Vol. 91-93, Issue 1-9 https://doi.org/10.1385/ABAB:91-93:1-9:5	journal	January 2001
Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues Lu, Yanpin; Yang, Bin; Gregg, David Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9 https://doi.org/10.1385/ABAB:98-100:1-9:641	journal	January 2002
The Reactions of Lignins with High Temperature Hydrogen Peroxide Part 2. The Oxidation of Kraft Lignin Kadla, J. F.; Chang, H. -m.; Jameel, H. Holzforschung, Vol. 53, Issue 3 https://doi.org/10.1515/HF.1999.047	journal	May 1999
Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography Minami, N. M.; Lucarini, A. C.; Kilikian, B. V. Brazilian Journal of Chemical Engineering, Vol. 16, Issue 2 https://doi.org/10.1590/S0104-66321999000200012	journal	June 1999

Similar Records

Lignin blockers and uses thereof

Patent · 2011 · OSTI ID:1013017

Lignin blockers and uses thereof

Patent · 2013 · OSTI ID:1107920

Related Subjects

60 APPLIED LIFE SCIENCES

Lignin-blocking treatment of biomass and uses thereof

Citation Formats

References (23)

Similar Records

Related Subjects