Rapid and Sensitive Detection of Protein Biomarker Using a Portable Fluorescence Biosensor based on Quantum Dots and a Lateral Flow Test Strip

Journal Article · Sun Aug 15 00:00:00 EDT 2010 · Analytical Chemistry, 82(16):7008-7014

DOI:https://doi.org/10.1021/ac101405a· OSTI ID:988633

Li, Zhaohui; Wang, Ying; Wang, Jun; Tang, Zhiwen; Pounds, Joel G; Lin, Yuehe

A portable fluorescence biosensor with rapid and ultrasensitive response for trace protein has been built up with quantum dots and lateral flow test strip. The superior signal brightness and high photostability of quantum dots are combined with the promising advantages of lateral flow test strip and resulted in high sensitivity, selectivity and speedy for protein detection. Nitrated ceruloplasmin, a significant biomarker for cardiovascular disease, lung cancer and stress response to smoking, was used as model protein to demonstrate the good performances of this proposed Qdot-based lateral flow test strip. Quantitative detection of nitrated ceruloplasmin was realized by recording the fluorescence intensity of quantum dots captured on the test line. Under optimal conditions, this portable fluorescence biosensor displays rapid responses for nitrated ceruloplasmin in wide dynamic range with a detection limit of 0.1ng/mL (S/N=3). Furthermore, the biosensor was successfully utilized for spiked human plasma sample detection with the concentration as low as 1ng/mL. The results demonstrate that the quantum dot-based lateral flow test strip is capable for rapid, sensitive, and quantitative detection of nitrated ceruloplasmin and hold a great promise for point-of-care and in field analysis of other protein biomarkers.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 988633
• Report Number(s): PNNL-SA-73545; 400412000; TRN: US201018%%591
• Journal Information: Analytical Chemistry, 82(16):7008-7014, Vol. 82, Issue 16
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
BRIGHTNESS
CARDIOVASCULAR DISEASES
CERULOPLASMIN
DETECTION
FLUORESCENCE
LUNGS
NEOPLASMS
PLASMA
PROTEINS
QUANTUM DOTS
SENSITIVITY
Quantum dots
strip
biosensor
nitrated protein