Structure, inheritance, and transcriptional effects of Pce1, an insertional element within Phanerochaete chrysosporium lignin peroxidase gene lipI
- Department of Agriculture, Madison, WI (United States)
- Univ. of Wisconsin, Madison, WI (United States)
- U.S. Department of Agriculture, Madison, WI (United States)
A 1747-bp insertion within a lignin peroxidase allele of Phanerochaete chrysosporium BKM-F-1767 is described. Pce1, the element, lies immediately adjacent to the fourth intron of lipI2. Southern blots reveal the presence of Pce1-homologous sequences in other P. chrysosporium strains. Transposon-like features include inverted terminal repeats and a dinucleotide (TA) target duplication. Atypical of transposons, Pce1 is present at very low copy numbers (one to five copies), and conserved transposase motifs are lacking. The mutation transcriptionally inactivates lipI2 and is inherited in a 1:1 Mendelian fashion among haploid progeny. Thus, Pce1 is a transposon-like element that may play a significant role in generating ligninolytic variation in certain P. chrysosporium strains. 39 refs., 7 figs.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-87ER13712
- OSTI ID:
- 98857
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16; Other Information: PBD: 1 Aug 1995
- Country of Publication:
- United States
- Language:
- English
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