Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Repressor-mediated tissue-specific gene expression in plants

Patent ·
OSTI ID:988296
 [1];  [2];  [1];  [1]
  1. Athens, GA
  2. Oxford, OH
+ Show Author Affiliations

Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.

Research Organization:
University of Georgia Research Foundation, Inc. (Athens, GA)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG07-02ER63493
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Number(s):
7,491,811
Application Number:
11/038,900
OSTI ID:
988296
Country of Publication:
United States
Language:
English

References (59)

nmt2 of fission yeast: A second thiamine-repressible gene co-ordinately regulated withnmt1 journal August 1994
Root-specific expression of theLeRse-1 gene in tomato is induced by exposure of the shoot to light journal October 1996
Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron journal January 1990
Characterization of Thiamin-Binding Protein from Buckwheat Seeds. journal January 1998
DNA-mediated genetic correction of thiamineless Arabidopsis thaliana journal May 1974
Development of transgenic yellow poplar for mercury phytoremediation journal October 1998
Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues journal July 1996
Tight-binding repressors of the lac operon: selection system and in vitro analysis. journal January 1979
Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana journal July 2001
An mRNA Putatively Coding for an O-Methyltransferase Accumulates Preferentially in Maize Roots and Is Located Predominantly in the Region of the Endodermis journal July 1993
Tapetum-Specific Expression of the Gene for an Endo-β-1,3-glucanase Causes Male Sterility in Transgenic Tobacco journal April 1995
A constitutive thiamine metabolism mutation, thi80, causing reduced thiamine pyrophosphokinase activity in Saccharomyces cerevisiae. journal January 1991
A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants journal June 1992
Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 or RNS2 Elevates Anthocyanin Accumulation journal January 1999
Phytoremediation of methylmercury pollution: merB expression in Arabidopsis thaliana confers resistance to organomercurials journal June 1999
Codon usage in plant genes journal January 1989
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana journal December 2000
Base substitution mutants of the lac operator: in vivo and in vitro affinities for lac repressor journal January 1986
Arabidopsis Contains Ancient Classes of Differentially Expressed Actin-Related Protein Genes journal March 2002
Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development journal May 2001
Characterization of cis-acting sequences regulating root-specific gene expression in tobacco. journal April 1991
Thiamin-Binding Protein from Sunflower Seeds. journal January 1998
Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants. journal September 1995
Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors journal November 1997
Operator DNA Sequence Variation Enhances High Affinity Binding by Hinge Helix Mutants of Lactose Repressor Protein † journal September 2000
The late pollen-specific actins in angiosperms journal June 1999
Phytodetoxification of hazardous organomercurials by genetically engineered plants journal February 2000
Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes journal June 1994
Phytoremediation of toxic elemental and organic pollutants journal April 2000
Dual role for the yeast THI4 gene in thiamine biosynthesis and DNA damage tolerance journal October 1997
Control of gene expression in tobacco cells using a bacterial operator-repressor system. journal April 1992
Root-specific genes from tobacco and Arabidopsis homologous to an evolutionarily conserved gene family of membrane channel proteins journal January 1990
Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression journal October 2002
A pyramid of loci for partial resistance to Fusarium solani f. sp. glycines maintains Myo-inositol-1-phosphate synthase expression in soybean roots journal December 2002
Cell cycle-dependent association of Arabidopsis actin-related proteins AtARP4 and AtARP7 with the nucleus: Two nuclear ARPs of Arabidopsis journal February 2003
Thiamine mutants of the crucifer, Arabidopsis journal April 1969
The Pyruvate decarboxylase1 Gene of Arabidopsis Is Required during Anoxia But Not Other Environmental Stresses journal April 2003
5′ proximal sequences of a soybean ribulose-1, 5-bisphosphate carboxylase small subunit gene direct light and phytochrome controlled transcription journal January 1987
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana journal April 2000
Positive Selection for Male-Sterile Mutants of Arabidopsis Lacking Adenine Phosphoribosyl Transferase Activity journal April 1988
The Arabidopsis thaliana ACT4/ACT12 actin gene subclass is strongly expressed throughout pollen development journal August 1996
Mercuric ion reduction and resistance in transgenic Arabidopsis thaliana plants expressing a modified bacterial merA gene. journal April 1996
Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase journal April 1999
Transfer of the inducible lac repressor/operator system from Escherichia coli to a vaccinia virus expression vector. journal April 1989
Thi1, a thiamine biosynthetic gene inArabidopsis thaliana, complements bacterial defects in DNA repair journal June 1996
Fungal pathogen protection in potato by expression of a plant defensin peptide journal December 2000
Isolation of Transcriptionally Regulated Root-Specific Genes from Tobacco journal July 1990
Functional Nonequivalency of Actin Isovariants in Arabidopsis journal January 2002
Phytoremediation of Mercury- and Methylmercury-Polluted Soils Using Genetically Engineered Plants journal July 1998
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. journal December 1987
Subcellular Targeting of Methylmercury Lyase Enhances Its Specific Activity for Organic Mercury Detoxification in Plants journal January 2003
The Arabidopsis ACT7 Actin Gene Is Expressed in Rapidly Developing Tissues and Responds to Several External Stimuli journal July 1996
Bcp1, a gene required for male fertility in Arabidopsis. journal March 1995
Overexpression of a Novel Arabidopsis Gene Related to Putative Zinc-Transporter Genes from Animals Can Lead to Enhanced Zinc Resistance and Accumulation journal March 1999
A combination of derepression of the lac operator-repressor system with positive induction by glucocorticoid and metal ions provides a high-level-inducible gene expression system based on the human metallothionein-IIA promoter. journal December 1990
Light-Regulated and Cell-Specific Expression of Tomato rbcS-gusA and Rice rbcS-gusA Fusion Genes in Transgenic Rice journal July 1993
Synthetic peptides as nuclear localization signals journal August 1986
SCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem journal February 2003
Toxic Mercury Reduction and Remediation Using Transgenic Plants with a Modified Bacterial Gene journal July 1998

Similar Records

Related Subjects

60 APPLIED LIFE SCIENCES