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Title: Apparatus and method for tracking a molecule or particle in three dimensions

Patent ·
OSTI ID:986482

An apparatus and method were used to track the movement of fluorescent particles in three dimensions. Control software was used with the apparatus to implement a tracking algorithm for tracking the motion of the individual particles in glycerol/water mixtures. Monte Carlo simulations suggest that the tracking algorithms in combination with the apparatus may be used for tracking the motion of single fluorescent or fluorescently labeled biomolecules in three dimensions.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-06NA25396
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Number(s):
7,498,551
Application Number:
11/544,988
OSTI ID:
986482
Country of Publication:
United States
Language:
English

References (13)

Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects journal October 2005
Three-dimensional fluorescent particle tracking at micron-scale using a single camera journal February 2005
Positional and Temporal Accuracy of Single Molecule Tracking journal September 2000
[38] Versatile optical traps with feedback control book January 1998
Tracking of fluorescent molecules diffusing within membranes journal November 2000
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics journal January 2005
Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon journal October 1997
Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position journal September 1994
SINGLE-PARTICLE TRACKING:Applications to Membrane Dynamics journal June 1997
Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions journal December 2004
Tracking-FCS: Fluorescence correlation spectroscopy of individual particles journal January 2005
Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels journal November 1996
3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells journal April 2005

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