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Title: Changes in microbial community structure correlate with stressed operating conditions during start-up of a field-scale denitrifying fluidized bed reactor

Abstract

High levels of nitrate are present in groundwater migrating from the former waste disposal ponds at the Y-12 National Security Complex in Oak Ridge, TN. A field-scale denitrifying fluidized bed reactor (FBR) was designed, constructed, and operated with ethanol as an electron donor for the removal of nitrate. After inoculation, biofilms developed on the granular activated carbon particles. Changes in the bacterial community of the FBR were evaluated with clone libraries (n=500 partial sequences) of the small-subunit rRNA gene for samples taken over a 4-month start-up period. Early phases of start-up operation were characterized by a period of selection, followed by low diversity and predominance by Azoarcus-like sequences. Possible explanations were high pH and nutrient limitations. After amelioration of these conditions, diversification increased rapidly, with the appearance of Dechloromonas, Pseudomonas, and Hydrogenophaga sequences. Changes in NO{sub 3}, SO{sub 4}, and pH also likely contributed to shifts in community composition. The detection of sulfate-reducing-bacteria-like sequences closely related to Desulfovibrio and Desulfuromonas in the FBR have important implications for downstream applications at the field site.

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [4];  [5];  [4];  [2];  [1]
  1. Miami University, Oxford, OH
  2. Stanford University
  3. Texas A&M University
  4. ORNL
  5. University of Oklahoma, Norman
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
986456
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Microbiology and Biotechnology; Journal Volume: 71; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ACTIVATED CARBON; BINDING ENERGY; DESULFOVIBRIO; DETECTION; DIVERSIFICATION; ELECTRONS; ETHANOL; FLUIDIZED BED REACTORS; GENES; INOCULATION; NATIONAL SECURITY; NITRATES; NUTRIENTS; PONDS; PSEUDOMONAS; REMOVAL; START-UP; VALENCE; WASTE DISPOSAL

Citation Formats

Hwang, Chaichi, Wu, Wei-min, Gentry, Terry, Carroll, Sue L, Schadt, Christopher Warren, Watson, David B, Jardine, Philip M, Zhou, Jizhong, Hickey, Robert, Criddle, Craig, and Fields, Matthew Wayne. Changes in microbial community structure correlate with stressed operating conditions during start-up of a field-scale denitrifying fluidized bed reactor. United States: N. p., 2006. Web. doi:10.1007/s00253-005-0189-1.
Hwang, Chaichi, Wu, Wei-min, Gentry, Terry, Carroll, Sue L, Schadt, Christopher Warren, Watson, David B, Jardine, Philip M, Zhou, Jizhong, Hickey, Robert, Criddle, Craig, & Fields, Matthew Wayne. Changes in microbial community structure correlate with stressed operating conditions during start-up of a field-scale denitrifying fluidized bed reactor. United States. doi:10.1007/s00253-005-0189-1.
Hwang, Chaichi, Wu, Wei-min, Gentry, Terry, Carroll, Sue L, Schadt, Christopher Warren, Watson, David B, Jardine, Philip M, Zhou, Jizhong, Hickey, Robert, Criddle, Craig, and Fields, Matthew Wayne. Mon . "Changes in microbial community structure correlate with stressed operating conditions during start-up of a field-scale denitrifying fluidized bed reactor". United States. doi:10.1007/s00253-005-0189-1.
@article{osti_986456,
title = {Changes in microbial community structure correlate with stressed operating conditions during start-up of a field-scale denitrifying fluidized bed reactor},
author = {Hwang, Chaichi and Wu, Wei-min and Gentry, Terry and Carroll, Sue L and Schadt, Christopher Warren and Watson, David B and Jardine, Philip M and Zhou, Jizhong and Hickey, Robert and Criddle, Craig and Fields, Matthew Wayne},
abstractNote = {High levels of nitrate are present in groundwater migrating from the former waste disposal ponds at the Y-12 National Security Complex in Oak Ridge, TN. A field-scale denitrifying fluidized bed reactor (FBR) was designed, constructed, and operated with ethanol as an electron donor for the removal of nitrate. After inoculation, biofilms developed on the granular activated carbon particles. Changes in the bacterial community of the FBR were evaluated with clone libraries (n=500 partial sequences) of the small-subunit rRNA gene for samples taken over a 4-month start-up period. Early phases of start-up operation were characterized by a period of selection, followed by low diversity and predominance by Azoarcus-like sequences. Possible explanations were high pH and nutrient limitations. After amelioration of these conditions, diversification increased rapidly, with the appearance of Dechloromonas, Pseudomonas, and Hydrogenophaga sequences. Changes in NO{sub 3}, SO{sub 4}, and pH also likely contributed to shifts in community composition. The detection of sulfate-reducing-bacteria-like sequences closely related to Desulfovibrio and Desulfuromonas in the FBR have important implications for downstream applications at the field site.},
doi = {10.1007/s00253-005-0189-1},
journal = {Applied Microbiology and Biotechnology},
number = 5,
volume = 71,
place = {United States},
year = {Mon May 01 00:00:00 EDT 2006},
month = {Mon May 01 00:00:00 EDT 2006}
}