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Title: Structural Determinats Underlying Photoprotection in the Photoactive Orange Carotenoid Protein of Cyanobacteria

Abstract

The photoprotective processes of photosynthetic organisms involve the dissipation of excess absorbed light energy as heat. Photoprotection in cyanobacteria is mechanistically distinct from that in plants; it involves the Orange Carotenoid Protein (OCP), a water-soluble protein containing a single carotenoid. The OCP is a new member of the family of blue light photoactive proteins; blue-green light triggers the OCP-mediated photoprotective response. Here we report structural and functional characterization of the wildtype and two mutant forms of the OCP, from the model organism Synechocystis PCC6803. The structural analysis provides highresolution detail of the carotenoidprotein interactions that underlie the optical properties of the OCP, unique among carotenoid-proteins in binding a single pigment per polypeptide chain. Collectively, these data implicate several key amino acids in the function of the OCP and reveal that the photoconversion and photoprotective responses of the OCP to blue-green light can be decoupled.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Genomics Division
OSTI Identifier:
986243
Report Number(s):
LBNL-3812E
TRN: US201017%%162
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
The Journal of Biological Chemistry
Additional Journal Information:
Journal Name: The Journal of Biological Chemistry
Country of Publication:
United States
Language:
English
Subject:
99 GENERAL AND MISCELLANEOUS; AMINO ACIDS; CAROTENOIDS; CYANOBACTERIA; FUNCTIONALS; MUTANTS; OPTICAL PROPERTIES; ORANGES; PIGMENTS; POLYPEPTIDES; PROTEINS; microbiology, photoprotection, carotenoid protein, cyanobacteria

Citation Formats

Wilson, Adjele, Kinney, James N, Zwart, Petrus H, Punginelli, Claire, D'Haene, Sandrine, Perreau, Francois, Klein, Michael G, Kirilovsky, Diana, and Kerfeld, Cheryl. Structural Determinats Underlying Photoprotection in the Photoactive Orange Carotenoid Protein of Cyanobacteria. United States: N. p., 2010. Web.
Wilson, Adjele, Kinney, James N, Zwart, Petrus H, Punginelli, Claire, D'Haene, Sandrine, Perreau, Francois, Klein, Michael G, Kirilovsky, Diana, & Kerfeld, Cheryl. Structural Determinats Underlying Photoprotection in the Photoactive Orange Carotenoid Protein of Cyanobacteria. United States.
Wilson, Adjele, Kinney, James N, Zwart, Petrus H, Punginelli, Claire, D'Haene, Sandrine, Perreau, Francois, Klein, Michael G, Kirilovsky, Diana, and Kerfeld, Cheryl. Thu . "Structural Determinats Underlying Photoprotection in the Photoactive Orange Carotenoid Protein of Cyanobacteria". United States. https://www.osti.gov/servlets/purl/986243.
@article{osti_986243,
title = {Structural Determinats Underlying Photoprotection in the Photoactive Orange Carotenoid Protein of Cyanobacteria},
author = {Wilson, Adjele and Kinney, James N and Zwart, Petrus H and Punginelli, Claire and D'Haene, Sandrine and Perreau, Francois and Klein, Michael G and Kirilovsky, Diana and Kerfeld, Cheryl},
abstractNote = {The photoprotective processes of photosynthetic organisms involve the dissipation of excess absorbed light energy as heat. Photoprotection in cyanobacteria is mechanistically distinct from that in plants; it involves the Orange Carotenoid Protein (OCP), a water-soluble protein containing a single carotenoid. The OCP is a new member of the family of blue light photoactive proteins; blue-green light triggers the OCP-mediated photoprotective response. Here we report structural and functional characterization of the wildtype and two mutant forms of the OCP, from the model organism Synechocystis PCC6803. The structural analysis provides highresolution detail of the carotenoidprotein interactions that underlie the optical properties of the OCP, unique among carotenoid-proteins in binding a single pigment per polypeptide chain. Collectively, these data implicate several key amino acids in the function of the OCP and reveal that the photoconversion and photoprotective responses of the OCP to blue-green light can be decoupled.},
doi = {},
journal = {The Journal of Biological Chemistry},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {4}
}