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Title: System and method for detecting cells or components thereof

Abstract

A system and method for detecting a detectably labeled cell or component thereof in a sample comprising one or more cells or components thereof, at least one cell or component thereof of which is detectably labeled with at least two detectable labels. In one embodiment, the method comprises: (i) introducing the sample into one or more flow cells of a flow cytometer, (ii) irradiating the sample with one or more light sources that are absorbed by the at least two detectable labels, the absorption of which is to be detected, and (iii) detecting simultaneously the absorption of light by the at least two detectable labels on the detectably labeled cell or component thereof with an array of photomultiplier tubes, which are operably linked to two or more filters that selectively transmit detectable emissions from the at least two detectable labels.

Inventors:
 [1];  [1];  [1];  [2];  [1]
  1. Ames, IA
  2. Corona, CA
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
985860
Patent Number(s):
7,473,529
Application Number:
11/095,098
Assignee:
Iowa State University Research Foundation, Inc. (Ames, IA) AMES
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Porter, Marc D, Lipert, Robert J, Doyle, Robert T, Grubisha, Desiree S, and Rahman, Salma. System and method for detecting cells or components thereof. United States: N. p., 2009. Web.
Porter, Marc D, Lipert, Robert J, Doyle, Robert T, Grubisha, Desiree S, & Rahman, Salma. System and method for detecting cells or components thereof. United States.
Porter, Marc D, Lipert, Robert J, Doyle, Robert T, Grubisha, Desiree S, and Rahman, Salma. Tue . "System and method for detecting cells or components thereof". United States. https://www.osti.gov/servlets/purl/985860.
@article{osti_985860,
title = {System and method for detecting cells or components thereof},
author = {Porter, Marc D and Lipert, Robert J and Doyle, Robert T and Grubisha, Desiree S and Rahman, Salma},
abstractNote = {A system and method for detecting a detectably labeled cell or component thereof in a sample comprising one or more cells or components thereof, at least one cell or component thereof of which is detectably labeled with at least two detectable labels. In one embodiment, the method comprises: (i) introducing the sample into one or more flow cells of a flow cytometer, (ii) irradiating the sample with one or more light sources that are absorbed by the at least two detectable labels, the absorption of which is to be detected, and (iii) detecting simultaneously the absorption of light by the at least two detectable labels on the detectably labeled cell or component thereof with an array of photomultiplier tubes, which are operably linked to two or more filters that selectively transmit detectable emissions from the at least two detectable labels.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {1}
}

Patent:

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