skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Field Evidence for Co-Metabolism of Trichloroethene Stimulated by Addition of Electron Donor to Groundwater

Abstract

For more than 10 years, electron donor has been injected into the Snake River aquifer beneath the Test Area North site of the Idaho National Laboratory for the purpose of stimulating microbial reductive dechlorination of trichloroethene (TCE) in groundwater. This has resulted in significant TCE removal from the source area of the contaminant plume and elevated dissolved CH4 in the groundwater extending 250 m from the injection well. The delta13C of the CH4 increases from 56o/oo in the source area to -13 o/oo with distance from the injection well, whereas the delta13C of dissolved inorganic carbon decreases from 8 o/oo to -13 o/oo, indicating a shift from methanogenesis to methane oxidation. This change in microbial activity along the plume axis is confirmed by PhyloChip microarray analyses of 16S rRNA genes obtained from groundwater microbial communities, which indicate decreasing abundances of reductive dechlorinating microorganisms (e.g., Dehalococcoides ethenogenes) and increasing CH4-oxidizing microorganisms capable of aerobic co-metabolism of TCE (e.g., Methylosinus trichosporium). Incubation experiments with 13C-labeled TCE introduced into microcosms containing basalt and groundwater from the aquifer confirm that TCE co-metabolism is possible. The results of these studies indicate that electron donor amendment designed to stimulate reductive dechlorination of TCE may also stimulatemore » co-metabolism of TCE.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Earth Sciences Division
OSTI Identifier:
985728
Report Number(s):
LBNL-3683E
Journal ID: ISSN 0013-936X; ESTHAG; TRN: US201016%%2103
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Environmental Science&Technology
Additional Journal Information:
Journal Volume: 44; Journal Issue: 12; Related Information: Journal Publication Date: May 17, 2010 (on-line publication); Journal ID: ISSN 0013-936X
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 58 GEOSCIENCES; AQUIFERS; BASALT; BINDING ENERGY; CARBON; DECHLORINATION; ELECTRONS; GENES; INCUBATION; INJECTION WELLS; METHANE; MICROCOSMS; MICROORGANISMS; OXIDATION; PLUMES; REMOVAL; VALENCE; GROUND WATER

Citation Formats

Conrad, Mark E, Brodie, Eoin L, Radtke, Corey W, Bill, Markus, Delwiche, Mark E, Lee, M Hope, Swift, Dana L, and Colwell, Frederick S. Field Evidence for Co-Metabolism of Trichloroethene Stimulated by Addition of Electron Donor to Groundwater. United States: N. p., 2010. Web. doi:10.1021/es903535j.
Conrad, Mark E, Brodie, Eoin L, Radtke, Corey W, Bill, Markus, Delwiche, Mark E, Lee, M Hope, Swift, Dana L, & Colwell, Frederick S. Field Evidence for Co-Metabolism of Trichloroethene Stimulated by Addition of Electron Donor to Groundwater. United States. doi:10.1021/es903535j.
Conrad, Mark E, Brodie, Eoin L, Radtke, Corey W, Bill, Markus, Delwiche, Mark E, Lee, M Hope, Swift, Dana L, and Colwell, Frederick S. Mon . "Field Evidence for Co-Metabolism of Trichloroethene Stimulated by Addition of Electron Donor to Groundwater". United States. doi:10.1021/es903535j. https://www.osti.gov/servlets/purl/985728.
@article{osti_985728,
title = {Field Evidence for Co-Metabolism of Trichloroethene Stimulated by Addition of Electron Donor to Groundwater},
author = {Conrad, Mark E and Brodie, Eoin L and Radtke, Corey W and Bill, Markus and Delwiche, Mark E and Lee, M Hope and Swift, Dana L and Colwell, Frederick S},
abstractNote = {For more than 10 years, electron donor has been injected into the Snake River aquifer beneath the Test Area North site of the Idaho National Laboratory for the purpose of stimulating microbial reductive dechlorination of trichloroethene (TCE) in groundwater. This has resulted in significant TCE removal from the source area of the contaminant plume and elevated dissolved CH4 in the groundwater extending 250 m from the injection well. The delta13C of the CH4 increases from 56o/oo in the source area to -13 o/oo with distance from the injection well, whereas the delta13C of dissolved inorganic carbon decreases from 8 o/oo to -13 o/oo, indicating a shift from methanogenesis to methane oxidation. This change in microbial activity along the plume axis is confirmed by PhyloChip microarray analyses of 16S rRNA genes obtained from groundwater microbial communities, which indicate decreasing abundances of reductive dechlorinating microorganisms (e.g., Dehalococcoides ethenogenes) and increasing CH4-oxidizing microorganisms capable of aerobic co-metabolism of TCE (e.g., Methylosinus trichosporium). Incubation experiments with 13C-labeled TCE introduced into microcosms containing basalt and groundwater from the aquifer confirm that TCE co-metabolism is possible. The results of these studies indicate that electron donor amendment designed to stimulate reductive dechlorination of TCE may also stimulate co-metabolism of TCE.},
doi = {10.1021/es903535j},
journal = {Environmental Science&Technology},
issn = {0013-936X},
number = 12,
volume = 44,
place = {United States},
year = {2010},
month = {5}
}