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Title: Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis

Abstract

Xylogalacturonan (XGA) is a class of pectic polysaccharide found in plant cell walls. The Arabidopsis thaliana locus At5g33290 encodes a predicted Type II membrane protein, and insertion mutants of the At5g33290 locus had decreased cell wall xylose. Immunological studies, enzymatic extraction of polysaccharides, monosaccharide linkage analysis, and oligosaccharide mass profiling were employed to identify the affected cell wall polymer. Pectic XGA was reduced to much lower levels in mutant than in wild-type leaves, indicating a role of At5g33290 in XGA biosynthesis. The mutated gene was designated xylogalacturonan deficient1 (xgd1). Transformation of the xgd1-1 mutant with the wild-type gene restored XGA to wild-type levels. XGD1 protein heterologously expressed in Nicotiana benthamiana catalyzed the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. The products formed could be hydrolyzed with an XGA-specific hydrolase. These results confirm that the XGD1 protein is a XGA xylosyltransferase. The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Physical Biosciences Division
OSTI Identifier:
985727
Report Number(s):
LBNL-2504E
TRN: US201016%%2102
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Plant Cell
Additional Journal Information:
Journal Volume: 20; Journal Issue: (5); Related Information: Journal Publication Date: 2008
Country of Publication:
United States
Language:
English
Subject:
09; ARABIDOPSIS; BIOSYNTHESIS; CELL WALL; GENES; MEMBRANE PROTEINS; MONOSACCHARIDES; MUTANTS; NICOTIANA; OLIGOSACCHARIDES; PECTINS; PLANT CELLS; POLYSACCHARIDES; PROTEINS; TRANSFORMATIONS; XYLOSE

Citation Formats

Pauly, Markus, Sorensen, Susanne Oxenboll, Harholt, Jesper, Geshi, Naomi, Sakuragi, Yumiko, Moller, Isabel, Zandleven, Joris, Bernal, Adriana J, Jensen, Niels Bjerg, Sorensen, Charlotte, Jensen, Jacob K, Beldman, Gerrit, Willats, William G.T., and Scheller, Henrik. Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis. United States: N. p., 2009. Web.
Pauly, Markus, Sorensen, Susanne Oxenboll, Harholt, Jesper, Geshi, Naomi, Sakuragi, Yumiko, Moller, Isabel, Zandleven, Joris, Bernal, Adriana J, Jensen, Niels Bjerg, Sorensen, Charlotte, Jensen, Jacob K, Beldman, Gerrit, Willats, William G.T., & Scheller, Henrik. Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis. United States.
Pauly, Markus, Sorensen, Susanne Oxenboll, Harholt, Jesper, Geshi, Naomi, Sakuragi, Yumiko, Moller, Isabel, Zandleven, Joris, Bernal, Adriana J, Jensen, Niels Bjerg, Sorensen, Charlotte, Jensen, Jacob K, Beldman, Gerrit, Willats, William G.T., and Scheller, Henrik. Wed . "Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis". United States. https://www.osti.gov/servlets/purl/985727.
@article{osti_985727,
title = {Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis},
author = {Pauly, Markus and Sorensen, Susanne Oxenboll and Harholt, Jesper and Geshi, Naomi and Sakuragi, Yumiko and Moller, Isabel and Zandleven, Joris and Bernal, Adriana J and Jensen, Niels Bjerg and Sorensen, Charlotte and Jensen, Jacob K and Beldman, Gerrit and Willats, William G.T. and Scheller, Henrik},
abstractNote = {Xylogalacturonan (XGA) is a class of pectic polysaccharide found in plant cell walls. The Arabidopsis thaliana locus At5g33290 encodes a predicted Type II membrane protein, and insertion mutants of the At5g33290 locus had decreased cell wall xylose. Immunological studies, enzymatic extraction of polysaccharides, monosaccharide linkage analysis, and oligosaccharide mass profiling were employed to identify the affected cell wall polymer. Pectic XGA was reduced to much lower levels in mutant than in wild-type leaves, indicating a role of At5g33290 in XGA biosynthesis. The mutated gene was designated xylogalacturonan deficient1 (xgd1). Transformation of the xgd1-1 mutant with the wild-type gene restored XGA to wild-type levels. XGD1 protein heterologously expressed in Nicotiana benthamiana catalyzed the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. The products formed could be hydrolyzed with an XGA-specific hydrolase. These results confirm that the XGD1 protein is a XGA xylosyltransferase. The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis.},
doi = {},
journal = {Plant Cell},
number = (5),
volume = 20,
place = {United States},
year = {2009},
month = {8}
}