Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

Culbertson, Christopher T; Jacobson, Stephen C; McClain, Maxine A; Ramsey, J Michael

Title: Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

Patent · Tue Sep 02 00:00:00 EDT 2008

OSTI ID:985165

Culbertson, Christopher T ^[1]; Jacobson, Stephen C ^[2]; McClain, Maxine A ^[2]; Ramsey, J Michael ^[2]

Oak Ridge, TN
Knoxville, TN

Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 7,419,575

Application Number:: 10/858,096

OSTI ID:: 985165

Country of Publication:: United States

Language:: English

References (8)

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Characterization of Cellular Optoporation with Distance Soughayer, Joseph S.; Krasieva, Tatiana; Jacobson, Stephen C. Analytical Chemistry, Vol. 72, Issue 6 https://doi.org/10.1021/ac990982u	journal	March 2000
Effects of high electric fields on micro-organisms Sale, A. J. H.; Hamilton, W. A. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 163, Issue 1 https://doi.org/10.1016/0005-2736(68)90030-8	journal	August 1968
Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects Li, Paul C. H.; Harrison, D. Jed Analytical Chemistry, Vol. 69, Issue 8 https://doi.org/10.1021/ac9606564	journal	April 1997
Voltage-induced pore formation and hemolysis of human erythrocytes Kinosita, Kazuhiko; Tsong, Tian Yow Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 471, Issue 2 https://doi.org/10.1016/0005-2736(77)90252-8	journal	December 1977
Hemolysis of human erythrocytes by transient electric field. Kinosita, K.; Tsong, T. T. Proceedings of the National Academy of Sciences, Vol. 74, Issue 5 https://doi.org/10.1073/pnas.74.5.1923	journal	May 1977
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane) Duffy, David C.; McDonald, J. Cooper; Schueller, Olivier J. A. Analytical Chemistry, Vol. 70, Issue 23, p. 4974-4984 https://doi.org/10.1021/ac980656z	journal	October 1998

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