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Title: Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination

Abstract

Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

Inventors:
 [1];  [1];  [2];  [3]
  1. San Diego, CA
  2. Boston, MA
  3. La Jolla, CA
Publication Date:
Research Org.:
Scripps Research Insitute
Sponsoring Org.:
USDOE
OSTI Identifier:
984459
Patent Number(s):
7,399,619
Application Number:
11/137,850
Assignee:
CHO
DOE Contract Number:  
FG02-03ER46051
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Xie, Jianming, Wang, Lei, Wu, Ning, and Schultz, Peter G. Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination. United States: N. p., 2008. Web.
Xie, Jianming, Wang, Lei, Wu, Ning, & Schultz, Peter G. Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination. United States.
Xie, Jianming, Wang, Lei, Wu, Ning, and Schultz, Peter G. Tue . "Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination". United States. https://www.osti.gov/servlets/purl/984459.
@article{osti_984459,
title = {Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination},
author = {Xie, Jianming and Wang, Lei and Wu, Ning and Schultz, Peter G},
abstractNote = {Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {7}
}

Patent:

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