Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor

Maupin-Furlow, Julie A; Talarico, Lee Ann; Raj, Krishnan Chandra; Ingram, Lonnie O

Title: Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor

Patent · Tue Feb 05 00:00:00 EST 2008

OSTI ID:983106

Maupin-Furlow, Julie A ^[1]; Talarico, Lee Ann ^[1]; Raj, Krishnan Chandra ^[2]; Ingram, Lonnie O ^[1]

Gainesville, FL
Tamil Nadu, IN

The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.

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Research Organization:: University of Florida Research Foundation, Inc. (Gainesville, FL)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-96ER20222

Assignee:: University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Number(s):: 7,326,551

Application Number:: 10/136,960

OSTI ID:: 983106

Country of Publication:: United States

Language:: English

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