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Title: Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor

Abstract

The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.

Inventors:
 [1];  [1];  [2];  [1]
  1. Gainesville, FL
  2. Tamil Nadu, IN
Publication Date:
Research Org.:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Sponsoring Org.:
USDOE
OSTI Identifier:
983106
Patent Number(s):
7,326,551
Application Number:
10/136,960
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States: N. p., 2008. Web.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, & Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Tue . "Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor". United States. https://www.osti.gov/servlets/purl/983106.
@article{osti_983106,
title = {Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor},
author = {Maupin-Furlow, Julie A and Talarico, Lee Ann and Raj, Krishnan Chandra and Ingram, Lonnie O},
abstractNote = {The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {2}
}

Patent:

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