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Title: From Molecular to Macroscopic via the Rational Design of a Self-Assembled 3D DNA Crystal

Abstract

We live in a macroscopic three-dimensional (3D) world, but our best description of the structure of matter is at the atomic and molecular scale. Understanding the relationship between the two scales requires a bridge from the molecular world to the macroscopic world. Connecting these two domains with atomic precision is a central goal of the natural sciences, but it requires high spatial control of the 3D structure of matter1. The simplest practical route to producing precisely designed 3D macroscopic objects is to form a crystalline arrangement by self-assembly, because such a periodic array has only conceptually simple requirements: a motif that has a robust 3D structure, dominant affinity interactions between parts of the motif when it self-associates, and predictable structures for these affinity interactions. Fulfilling these three criteria to produce a 3D periodic system is not easy, but should readily be achieved with well-structured branched DNA motifs tailed by sticky ends2. Complementary sticky ends associate with each other preferentially and assume the well-known B-DNA structure when they do so3; the helically repeating nature of DNA facilitates the construction of a periodic array. It is essential that the directions of propagation associated with the sticky ends do not share the samemore » plane, but extend to form a 3D arrangement of matter. Here we report the crystal structure at 4?Angstroms resolution of a designed, self-assembled, 3D crystal based on the DNA tensegrity triangle4. The data demonstrate clearly that it is possible to design and self-assemble a well-ordered macromolecular 3D crystalline lattice with precise control.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
980215
Report Number(s):
BNL-93133-2010-JA
Journal ID: ISSN 0028-0836; NATUAS; TRN: US201015%%1600
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
Nature (London)
Additional Journal Information:
Journal Volume: 461; Journal ID: ISSN 0028-0836
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; ACCURACY; AFFINITY; CONSTRUCTION; CRYSTAL STRUCTURE; DESIGN; DNA; PERIODIC SYSTEM; RESOLUTION; CRYSTALS; national synchrotron light source

Citation Formats

Zheng, J, Birktoft, J, Yi, C, Tong, W, Ruojie, S, Constantinou, P, Ginell, S, Chenge, M, and Seeman, N. From Molecular to Macroscopic via the Rational Design of a Self-Assembled 3D DNA Crystal. United States: N. p., 2009. Web. doi:10.1038/nature08274.
Zheng, J, Birktoft, J, Yi, C, Tong, W, Ruojie, S, Constantinou, P, Ginell, S, Chenge, M, & Seeman, N. From Molecular to Macroscopic via the Rational Design of a Self-Assembled 3D DNA Crystal. United States. doi:10.1038/nature08274.
Zheng, J, Birktoft, J, Yi, C, Tong, W, Ruojie, S, Constantinou, P, Ginell, S, Chenge, M, and Seeman, N. Thu . "From Molecular to Macroscopic via the Rational Design of a Self-Assembled 3D DNA Crystal". United States. doi:10.1038/nature08274.
@article{osti_980215,
title = {From Molecular to Macroscopic via the Rational Design of a Self-Assembled 3D DNA Crystal},
author = {Zheng, J and Birktoft, J and Yi, C and Tong, W and Ruojie, S and Constantinou, P and Ginell, S and Chenge, M and Seeman, N},
abstractNote = {We live in a macroscopic three-dimensional (3D) world, but our best description of the structure of matter is at the atomic and molecular scale. Understanding the relationship between the two scales requires a bridge from the molecular world to the macroscopic world. Connecting these two domains with atomic precision is a central goal of the natural sciences, but it requires high spatial control of the 3D structure of matter1. The simplest practical route to producing precisely designed 3D macroscopic objects is to form a crystalline arrangement by self-assembly, because such a periodic array has only conceptually simple requirements: a motif that has a robust 3D structure, dominant affinity interactions between parts of the motif when it self-associates, and predictable structures for these affinity interactions. Fulfilling these three criteria to produce a 3D periodic system is not easy, but should readily be achieved with well-structured branched DNA motifs tailed by sticky ends2. Complementary sticky ends associate with each other preferentially and assume the well-known B-DNA structure when they do so3; the helically repeating nature of DNA facilitates the construction of a periodic array. It is essential that the directions of propagation associated with the sticky ends do not share the same plane, but extend to form a 3D arrangement of matter. Here we report the crystal structure at 4?Angstroms resolution of a designed, self-assembled, 3D crystal based on the DNA tensegrity triangle4. The data demonstrate clearly that it is possible to design and self-assemble a well-ordered macromolecular 3D crystalline lattice with precise control.},
doi = {10.1038/nature08274},
journal = {Nature (London)},
issn = {0028-0836},
number = ,
volume = 461,
place = {United States},
year = {2009},
month = {1}
}