Crystal Structure and Catalytic Mechanism of PglD from Campylobacter jejuni

Journal Article · Tue Jan 01 00:00:00 EST 2008 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.M801207200· OSTI ID:980092

Olivier, N; Imperiali, B

The carbohydrate 2, 4-diacetamido-2, 4, 6-trideoxy-{alpha}-d-glucopyranose (BacAc2) is found in a variety of eubacterial pathogens. In Campylobacter jejuni, PglD acetylates the C4 amino group on UDP-2-acetamido-4-amino-2, 4, 6-trideoxy-a-d-glucopyranose (UDP-4-amino-sugar) to form UDP-BacAc2. Sequence analysis predicts PglD to be a member of the left-handed {Beta} helix family of enzymes. However, poor sequence homology between PglD and left-handed {Beta} helix enzymes with existing structural data precludes unambiguous identification of the active site. The co-crystal structures of PglD in the presence of citrate, acetyl coenzyme A, or the UDP-4-amino-sugar were solved. The biological assembly is a trimer with one active site formed between two protomers. Residues lining the active site were identified, and results from functional assays on alanine mutants suggest His-125 is critical for catalysis, whereas His-15 and His-134 are involved in substrate binding. These results are discussed in the context of implications for proteins homologous to PglD in other pathogens.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: DE-AC02-98CH10886
• OSTI ID: 980092
• Report Number(s): BNL-93010-2010-JA; JBCHA3; TRN: US201015%%1477
• Journal Information: Journal of Biological Chemistry, Vol. 283, Issue 41; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ALANINES
CARBOHYDRATES
CATALYSIS
COENZYMES
CRYSTAL STRUCTURE
DATA
ENZYMES
FUNCTIONALS
LINERS
MUTANTS
PATHOGENS
PROTEINS
RESIDUES
STRUCTURAL CHEMICAL ANALYSIS
SUBSTRATES
national synchrotron light source