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Title: In Situ Probing of Cholesterol in Astrocytes at the Single Cell Level using Laser Desorption Ionization Mass Spectrometric Imaging with Colloidal Silver

Abstract

Mass spectrometric imaging has been utilized to localize individual astrocytes and to obtain cholesterol populations at the single-cell level in laser desorption ionization (LDI) with colloidal silver. The silver ion adduct of membrane-bound cholesterol was monitored to detect individual cells. Good correlation between mass spectrometric and optical images at different cell densities indicates the ability to perform single-cell studies of cholesterol abundance. The feasibility of quantification is confirmed by the agreement between the LDI-MS ion signals and the results from a traditional enzymatic fluorometric assay. We propose that this approach could be an effective tool to study chemical populations at the cellular level.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
976054
Report Number(s):
IS-J 7547
Journal ID: 0951-4198; TRN: US201009%%511
DOE Contract Number:  
DE-AC02-07CH11358
Resource Type:
Journal Article
Resource Relation:
Journal Name: Rapid Communications in Mass Spectrometry; Journal Volume: 24; Journal Issue: 8
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; ABUNDANCE; ADDUCTS; CHOLESTEROL; DESORPTION; IONIZATION; LASERS; SILVER; SILVER IONS

Citation Formats

Perdian, D.C., Cha, Sangwon, Oh, Jisun, Sakaguchi, Donald S., Yeung, Edward S., and and Lee, Young Jin. In Situ Probing of Cholesterol in Astrocytes at the Single Cell Level using Laser Desorption Ionization Mass Spectrometric Imaging with Colloidal Silver. United States: N. p., 2010. Web. doi:10.1002/rcm.4491.
Perdian, D.C., Cha, Sangwon, Oh, Jisun, Sakaguchi, Donald S., Yeung, Edward S., & and Lee, Young Jin. In Situ Probing of Cholesterol in Astrocytes at the Single Cell Level using Laser Desorption Ionization Mass Spectrometric Imaging with Colloidal Silver. United States. doi:10.1002/rcm.4491.
Perdian, D.C., Cha, Sangwon, Oh, Jisun, Sakaguchi, Donald S., Yeung, Edward S., and and Lee, Young Jin. Thu . "In Situ Probing of Cholesterol in Astrocytes at the Single Cell Level using Laser Desorption Ionization Mass Spectrometric Imaging with Colloidal Silver". United States. doi:10.1002/rcm.4491.
@article{osti_976054,
title = {In Situ Probing of Cholesterol in Astrocytes at the Single Cell Level using Laser Desorption Ionization Mass Spectrometric Imaging with Colloidal Silver},
author = {Perdian, D.C. and Cha, Sangwon and Oh, Jisun and Sakaguchi, Donald S. and Yeung, Edward S. and and Lee, Young Jin},
abstractNote = {Mass spectrometric imaging has been utilized to localize individual astrocytes and to obtain cholesterol populations at the single-cell level in laser desorption ionization (LDI) with colloidal silver. The silver ion adduct of membrane-bound cholesterol was monitored to detect individual cells. Good correlation between mass spectrometric and optical images at different cell densities indicates the ability to perform single-cell studies of cholesterol abundance. The feasibility of quantification is confirmed by the agreement between the LDI-MS ion signals and the results from a traditional enzymatic fluorometric assay. We propose that this approach could be an effective tool to study chemical populations at the cellular level.},
doi = {10.1002/rcm.4491},
journal = {Rapid Communications in Mass Spectrometry},
number = 8,
volume = 24,
place = {United States},
year = {Thu Mar 18 00:00:00 EDT 2010},
month = {Thu Mar 18 00:00:00 EDT 2010}
}