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Title: Metagenomic Analysis of Microbial Symbionts in a Gutless Worm

Abstract

Symbioses between bacteria and eukaryotes are ubiquitous, yet our understanding of the interactions driving these associations is hampered by our inability to cultivate most host-associated microbes. Here we use a metagenomic approach to describe four co-occurring symbionts from the marine oligochaete Olavius algarvensis, a worm lacking a mouth, gut and nephridia. Shotgun sequencing and metabolic pathway reconstruction revealed that the symbionts are sulphur-oxidizing and sulphate-reducing bacteria, all of which are capable of carbon fixation, thus providing the host with multiple sources of nutrition. Molecular evidence for the uptake and recycling of worm waste products by the symbionts suggests how the worm could eliminate its excretory system, an adaptation unique among annelid worms. We propose a model that describes how the versatile metabolism within this symbiotic consortium provides the host with an optimal energy supply as it shuttles between the upper oxic and lower anoxic coastal sediments that it inhabits.

Authors:
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Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Genomics Division
OSTI Identifier:
974268
Report Number(s):
LBNL-2723E
TRN: US201007%%364
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Nature
Additional Journal Information:
Journal Name: Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANNELIDS; AVAILABILITY; BACTERIA; BIOLOGICAL PATHWAYS; CARBON; METABOLISM; NUTRITION; RECYCLING; SEDIMENTS; WASTES

Citation Formats

Woyke, Tanja, Teeling, Hanno, Ivanova, Natalia N, Hunteman, Marcel, Richter, Michael, Gloeckner, Frank Oliver, Boeffelli, Dario, Barry, Kerrie W, Shapiro, Harris J, Anderson, Iain J, Szeto, Ernest, Kyrpides, Nikos C, Mussmann, Marc, Amann, Rudolf, Bergin, Claudia, Ruehland, Caroline, Rubin, Edward M, and Dubilier, Nicole. Metagenomic Analysis of Microbial Symbionts in a Gutless Worm. United States: N. p., 2006. Web. doi:10.1038/nature05192.
Woyke, Tanja, Teeling, Hanno, Ivanova, Natalia N, Hunteman, Marcel, Richter, Michael, Gloeckner, Frank Oliver, Boeffelli, Dario, Barry, Kerrie W, Shapiro, Harris J, Anderson, Iain J, Szeto, Ernest, Kyrpides, Nikos C, Mussmann, Marc, Amann, Rudolf, Bergin, Claudia, Ruehland, Caroline, Rubin, Edward M, & Dubilier, Nicole. Metagenomic Analysis of Microbial Symbionts in a Gutless Worm. United States. doi:10.1038/nature05192.
Woyke, Tanja, Teeling, Hanno, Ivanova, Natalia N, Hunteman, Marcel, Richter, Michael, Gloeckner, Frank Oliver, Boeffelli, Dario, Barry, Kerrie W, Shapiro, Harris J, Anderson, Iain J, Szeto, Ernest, Kyrpides, Nikos C, Mussmann, Marc, Amann, Rudolf, Bergin, Claudia, Ruehland, Caroline, Rubin, Edward M, and Dubilier, Nicole. Mon . "Metagenomic Analysis of Microbial Symbionts in a Gutless Worm". United States. doi:10.1038/nature05192. https://www.osti.gov/servlets/purl/974268.
@article{osti_974268,
title = {Metagenomic Analysis of Microbial Symbionts in a Gutless Worm},
author = {Woyke, Tanja and Teeling, Hanno and Ivanova, Natalia N and Hunteman, Marcel and Richter, Michael and Gloeckner, Frank Oliver and Boeffelli, Dario and Barry, Kerrie W and Shapiro, Harris J and Anderson, Iain J and Szeto, Ernest and Kyrpides, Nikos C and Mussmann, Marc and Amann, Rudolf and Bergin, Claudia and Ruehland, Caroline and Rubin, Edward M and Dubilier, Nicole},
abstractNote = {Symbioses between bacteria and eukaryotes are ubiquitous, yet our understanding of the interactions driving these associations is hampered by our inability to cultivate most host-associated microbes. Here we use a metagenomic approach to describe four co-occurring symbionts from the marine oligochaete Olavius algarvensis, a worm lacking a mouth, gut and nephridia. Shotgun sequencing and metabolic pathway reconstruction revealed that the symbionts are sulphur-oxidizing and sulphate-reducing bacteria, all of which are capable of carbon fixation, thus providing the host with multiple sources of nutrition. Molecular evidence for the uptake and recycling of worm waste products by the symbionts suggests how the worm could eliminate its excretory system, an adaptation unique among annelid worms. We propose a model that describes how the versatile metabolism within this symbiotic consortium provides the host with an optimal energy supply as it shuttles between the upper oxic and lower anoxic coastal sediments that it inhabits.},
doi = {10.1038/nature05192},
journal = {Nature},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {5}
}