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Title: GeoChip-based analysis of functional microbial communities in a bioreduced uranium-contaminated aquifer during reoxidation by oxygen

Abstract

A pilot-scale system was established for in situ biostimulation of U(VI) reduction by ethanol addition at the US Department of Energy's (DOE's) Field Research Center (Oak Ridge, TN). After achieving U(VI) reduction, stability of the bioreduced U(IV) was evaluated under conditions of (i) resting (no ethanol injection), (ii) reoxidation by introducing dissolved oxygen (DO), and (iii) reinjection of ethanol. GeoChip, a functional gene array with probes for N, S and C cycling, metal resistance and contaminant degradation genes, was used for monitoring groundwater microbial communities. High diversity of all major functional groups was observed during all experimental phases. The microbial community was extremely responsive to ethanol, showing a substantial change in community structure with increased gene number and diversity after ethanol injections resumed. While gene numbers showed considerable variations, the relative abundance (i.e. percentage of each gene category) of most gene groups changed little. During the reoxidation period, U(VI) increased, suggesting reoxidation of reduced U(IV). However, when introduction of DO was stopped, U(VI) reduction resumed and returned to pre-reoxidation levels. These findings suggest that the community in this system can be stimulated and that the ability to reduce U(VI) can be maintained by the addition of electron donors. This biostimulationmore » approach may potentially offer an effective means for the bioremediation of U(VI)-contaminated sites.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Earth Sciences Division
OSTI Identifier:
973686
Report Number(s):
LBNL-2636E
Journal ID: ISSN 1462-2912; TRN: US201006%%1059
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Microbiology; Journal Volume: 11; Journal Issue: 10; Related Information: Journal Publication Date: 2009
Country of Publication:
United States
Language:
English
Subject:
54; 58; ABUNDANCE; AQUIFERS; BINDING ENERGY; BIOREMEDIATION; COMMUNITIES; DISSOLVED GASES; ELECTRONS; ETHANOL; FUNCTIONALS; GENES; MONITORING; OXYGEN; PROBES; REINJECTION; STABILITY; VALENCE

Citation Formats

Van Nostrand, J.D., Wu, W.-M., Wu, L., Deng, Y., Carley, J., Carroll, S., He, Z., Gu, B., Luo, J., Criddle, C. S., Watson, D. B., Jardine, P. M., Tiedje, J. M., Hazen, T. C., and Zhou, J. GeoChip-based analysis of functional microbial communities in a bioreduced uranium-contaminated aquifer during reoxidation by oxygen. United States: N. p., 2009. Web. doi:10.1111/j.1462-2920.2009.01986.x.
Van Nostrand, J.D., Wu, W.-M., Wu, L., Deng, Y., Carley, J., Carroll, S., He, Z., Gu, B., Luo, J., Criddle, C. S., Watson, D. B., Jardine, P. M., Tiedje, J. M., Hazen, T. C., & Zhou, J. GeoChip-based analysis of functional microbial communities in a bioreduced uranium-contaminated aquifer during reoxidation by oxygen. United States. doi:10.1111/j.1462-2920.2009.01986.x.
Van Nostrand, J.D., Wu, W.-M., Wu, L., Deng, Y., Carley, J., Carroll, S., He, Z., Gu, B., Luo, J., Criddle, C. S., Watson, D. B., Jardine, P. M., Tiedje, J. M., Hazen, T. C., and Zhou, J. Wed . "GeoChip-based analysis of functional microbial communities in a bioreduced uranium-contaminated aquifer during reoxidation by oxygen". United States. doi:10.1111/j.1462-2920.2009.01986.x. https://www.osti.gov/servlets/purl/973686.
@article{osti_973686,
title = {GeoChip-based analysis of functional microbial communities in a bioreduced uranium-contaminated aquifer during reoxidation by oxygen},
author = {Van Nostrand, J.D. and Wu, W.-M. and Wu, L. and Deng, Y. and Carley, J. and Carroll, S. and He, Z. and Gu, B. and Luo, J. and Criddle, C. S. and Watson, D. B. and Jardine, P. M. and Tiedje, J. M. and Hazen, T. C. and Zhou, J.},
abstractNote = {A pilot-scale system was established for in situ biostimulation of U(VI) reduction by ethanol addition at the US Department of Energy's (DOE's) Field Research Center (Oak Ridge, TN). After achieving U(VI) reduction, stability of the bioreduced U(IV) was evaluated under conditions of (i) resting (no ethanol injection), (ii) reoxidation by introducing dissolved oxygen (DO), and (iii) reinjection of ethanol. GeoChip, a functional gene array with probes for N, S and C cycling, metal resistance and contaminant degradation genes, was used for monitoring groundwater microbial communities. High diversity of all major functional groups was observed during all experimental phases. The microbial community was extremely responsive to ethanol, showing a substantial change in community structure with increased gene number and diversity after ethanol injections resumed. While gene numbers showed considerable variations, the relative abundance (i.e. percentage of each gene category) of most gene groups changed little. During the reoxidation period, U(VI) increased, suggesting reoxidation of reduced U(IV). However, when introduction of DO was stopped, U(VI) reduction resumed and returned to pre-reoxidation levels. These findings suggest that the community in this system can be stimulated and that the ability to reduce U(VI) can be maintained by the addition of electron donors. This biostimulation approach may potentially offer an effective means for the bioremediation of U(VI)-contaminated sites.},
doi = {10.1111/j.1462-2920.2009.01986.x},
journal = {Environmental Microbiology},
number = 10,
volume = 11,
place = {United States},
year = {Wed Jul 15 00:00:00 EDT 2009},
month = {Wed Jul 15 00:00:00 EDT 2009}
}