Cetyltrimethylammonium Bromide-Modified Spherical and Cube-Like Gold Nanoparticles as Extrinsic Raman Lables in Surface-Enhanced Raman Spectroscopy Based Heterogeneous Immunoassays

doi:https://doi.org/10.1021/ac7026436

Title: Cetyltrimethylammonium Bromide-Modified Spherical and Cube-Like Gold Nanoparticles as Extrinsic Raman Lables in Surface-Enhanced Raman Spectroscopy Based Heterogeneous Immunoassays

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Abstract

This paper reports on the characterization and preliminary comparison of gold nanoparticles of differing surface modification and shape when used as extrinsic Raman labels (ERLs) in high-sensitivity heterogeneous immunoassays based on surface enhanced Raman scattering (SERS). ERLs are gold nanoparticles coated with an adlayer of an intrinsically strong Raman scatterer, followed by a coating of a molecular recognition element (e.g., antibody). Three types of ERLs, all with a nominal size of {approx}30 nm, were fabricated by using spherical citrate-capped gold nanoparticles (sp-cit-Au NPs), spherical CTAB-capped gold nanoparticles (sp-CTAB-Au NPs), or cube-like CTAB-capped gold nanoparticles (cu-CTAB-Au NPs) as cores. The performance of these particles was assessed via a sandwich immunoassay for human IgG in phosphate buffered saline. The ERLs fabricated with sp-CTAB-Au NPs as cores proved to be more than 50 times more sensitive than those with sp-cit-Au NPs as cores; the same comparison showed that the ERLs with cu-CTAB-Au NPs as cores were close to 200 times more sensitive. Coupled with small differences in levels of nonspecific adsorption, these sensitivities translated to a limit of detection (LOD) of 94, 2.3, and 0.28 ng/mL, respectively, for the detection of human IgG in the case of sp-cit-Au NPs, sp-CTAB-Au NPs, and cu-CTAB-Aumore »« less

Authors:: Narayanan, R; Lipert, R; Porter, M

Publication Date:: 2008-02-22

Research Org.:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 973390

Report Number(s):: IS-J 7388
Journal ID: 0003-2700; TRN: US201006%%361

DOE Contract Number:: DE-AC02-07CH11358

Resource Type:: Journal Article

Journal Name:: Analytical Chemistry

Additional Journal Information:: Journal Volume: 80; Journal Issue: 6

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; ADSORPTION; COATINGS; DETECTION; GOLD; IMMUNOASSAY; MODIFICATIONS; PERFORMANCE; PHOSPHATES; RAMAN SPECTROSCOPY; SCATTERING; SHAPE

Citation Formats


                    Narayanan, R, Lipert, R, and Porter, M. Cetyltrimethylammonium Bromide-Modified Spherical and Cube-Like Gold Nanoparticles as Extrinsic Raman Lables in Surface-Enhanced Raman Spectroscopy Based Heterogeneous Immunoassays.  United States: N. p., 2008. 
        Web.  doi:10.1021/ac7026436.

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                    Narayanan, R, Lipert, R, & Porter, M. Cetyltrimethylammonium Bromide-Modified Spherical and Cube-Like Gold Nanoparticles as Extrinsic Raman Lables in Surface-Enhanced Raman Spectroscopy Based Heterogeneous Immunoassays.  United States.  https://doi.org/10.1021/ac7026436

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                    Narayanan, R, Lipert, R, and Porter, M. Fri .  
        "Cetyltrimethylammonium Bromide-Modified Spherical and Cube-Like Gold Nanoparticles as Extrinsic Raman Lables in Surface-Enhanced Raman Spectroscopy Based Heterogeneous Immunoassays".  United States.  https://doi.org/10.1021/ac7026436.

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@article{osti_973390,

  title        = {Cetyltrimethylammonium Bromide-Modified Spherical and Cube-Like Gold Nanoparticles as Extrinsic Raman Lables in Surface-Enhanced Raman Spectroscopy Based Heterogeneous Immunoassays},

  author       = {Narayanan, R and Lipert, R and Porter, M},

  abstractNote = {This paper reports on the characterization and preliminary comparison of gold nanoparticles of differing surface modification and shape when used as extrinsic Raman labels (ERLs) in high-sensitivity heterogeneous immunoassays based on surface enhanced Raman scattering (SERS). ERLs are gold nanoparticles coated with an adlayer of an intrinsically strong Raman scatterer, followed by a coating of a molecular recognition element (e.g., antibody). Three types of ERLs, all with a nominal size of {approx}30 nm, were fabricated by using spherical citrate-capped gold nanoparticles (sp-cit-Au NPs), spherical CTAB-capped gold nanoparticles (sp-CTAB-Au NPs), or cube-like CTAB-capped gold nanoparticles (cu-CTAB-Au NPs) as cores. The performance of these particles was assessed via a sandwich immunoassay for human IgG in phosphate buffered saline. The ERLs fabricated with sp-CTAB-Au NPs as cores proved to be more than 50 times more sensitive than those with sp-cit-Au NPs as cores; the same comparison showed that the ERLs with cu-CTAB-Au NPs as cores were close to 200 times more sensitive. Coupled with small differences in levels of nonspecific adsorption, these sensitivities translated to a limit of detection (LOD) of 94, 2.3, and 0.28 ng/mL, respectively, for the detection of human IgG in the case of sp-cit-Au NPs, sp-CTAB-Au NPs, and cu-CTAB-Au NPs. The LOD of the cu-CTAB-Au NPs is therefore {approx}340 times below that for the sp-cit-Au NPs. Potential applications of these labels to bioassays are briefly discussed.},

  doi          = {10.1021/ac7026436},

  url          = {https://www.osti.gov/biblio/973390},
  journal      = {Analytical Chemistry},
number       = 6,

  volume       = 80,

  place        = {United States},

  year         = {2008},

  month        = {2}

}

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