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Title: Protein subcellular localization assays using split fluorescent proteins

Abstract

The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

Inventors:
 [1];  [2]
  1. Santa Fe, NM
  2. Los Alamos, NM
Publication Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Sponsoring Org.:
USDOE
OSTI Identifier:
971623
Patent Number(s):
7,585,636
Application Number:
11/295,374
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM) ALO
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Waldo, Geoffrey S, and Cabantous, Stephanie. Protein subcellular localization assays using split fluorescent proteins. United States: N. p., 2009. Web.
Waldo, Geoffrey S, & Cabantous, Stephanie. Protein subcellular localization assays using split fluorescent proteins. United States.
Waldo, Geoffrey S, and Cabantous, Stephanie. Tue . "Protein subcellular localization assays using split fluorescent proteins". United States. https://www.osti.gov/servlets/purl/971623.
@article{osti_971623,
title = {Protein subcellular localization assays using split fluorescent proteins},
author = {Waldo, Geoffrey S and Cabantous, Stephanie},
abstractNote = {The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {9}
}

Patent:

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