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Title: Wavelength-Dependent Differential Interference Contrast Microscopy: Selectively Imaging Nanoparticle Probes in Live Cells

Abstract

Gold and silver nanoparticles display extraordinarily large apparent refractive indices near their plasmon resonance (PR) wavelengths. These nanoparticles show good contrast in a narrow spectral band but are poorly resolved at other wavelengths in differential interference contrast (DIC) microscopy. The wavelength dependence of DIC contrast of gold/silver nanoparticles is interpreted in terms of Mie's theory and DIC working principles. We further exploit this wavelength dependence by modifying a DIC microscope to enable simultaneous imaging at two wavelengths. We demonstrate that gold/silver nanoparticles immobilized on the same glass slides through hybridization can be differentiated and imaged separately. High-contrast, video-rate images of living cells can be recorded both with and without illuminating the gold nanoparticle probes, providing definitive probe identification. Dual-wavelength DIC microscopy thus presents a new approach to the simultaneous detection of multiple probes of interest for high-speed live-cell imaging.

Authors:
; ; ;
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
969024
Report Number(s):
IS-J 7512
Journal ID: 0003-2700; TRN: US201001%%307
DOE Contract Number:  
DE-AC02-07CH11358
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 81; Journal Issue: 22
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; DETECTION; FREQUENCY DEPENDENCE; GLASS; GOLD; HYBRIDIZATION; MICROSCOPES; MICROSCOPY; PLASMONS; PROBES; RESONANCE; SILVER; WAVELENGTHS

Citation Formats

Sun, Wei, Wang, Gufeng, Fang, Ning, and and Yeung, Edward S. Wavelength-Dependent Differential Interference Contrast Microscopy: Selectively Imaging Nanoparticle Probes in Live Cells. United States: N. p., 2009. Web. doi:10.1021/ac901623b.
Sun, Wei, Wang, Gufeng, Fang, Ning, & and Yeung, Edward S. Wavelength-Dependent Differential Interference Contrast Microscopy: Selectively Imaging Nanoparticle Probes in Live Cells. United States. https://doi.org/10.1021/ac901623b
Sun, Wei, Wang, Gufeng, Fang, Ning, and and Yeung, Edward S. Sun . "Wavelength-Dependent Differential Interference Contrast Microscopy: Selectively Imaging Nanoparticle Probes in Live Cells". United States. https://doi.org/10.1021/ac901623b.
@article{osti_969024,
title = {Wavelength-Dependent Differential Interference Contrast Microscopy: Selectively Imaging Nanoparticle Probes in Live Cells},
author = {Sun, Wei and Wang, Gufeng and Fang, Ning and and Yeung, Edward S},
abstractNote = {Gold and silver nanoparticles display extraordinarily large apparent refractive indices near their plasmon resonance (PR) wavelengths. These nanoparticles show good contrast in a narrow spectral band but are poorly resolved at other wavelengths in differential interference contrast (DIC) microscopy. The wavelength dependence of DIC contrast of gold/silver nanoparticles is interpreted in terms of Mie's theory and DIC working principles. We further exploit this wavelength dependence by modifying a DIC microscope to enable simultaneous imaging at two wavelengths. We demonstrate that gold/silver nanoparticles immobilized on the same glass slides through hybridization can be differentiated and imaged separately. High-contrast, video-rate images of living cells can be recorded both with and without illuminating the gold nanoparticle probes, providing definitive probe identification. Dual-wavelength DIC microscopy thus presents a new approach to the simultaneous detection of multiple probes of interest for high-speed live-cell imaging.},
doi = {10.1021/ac901623b},
url = {https://www.osti.gov/biblio/969024}, journal = {Analytical Chemistry},
number = 22,
volume = 81,
place = {United States},
year = {2009},
month = {11}
}