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Title: High throughput protein production screening

Abstract

Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

Inventors:
 [1];  [2];  [3]
  1. Walnut Creek, CA
  2. Oakland, CA
  3. San Ramon, CA
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
968305
Patent Number(s):
7,585,815
Application Number:
10/884,783
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) OAK
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Beernink, Peter T, Coleman, Matthew A, and Segelke, Brent W. High throughput protein production screening. United States: N. p., 2009. Web.
Beernink, Peter T, Coleman, Matthew A, & Segelke, Brent W. High throughput protein production screening. United States.
Beernink, Peter T, Coleman, Matthew A, and Segelke, Brent W. Tue . "High throughput protein production screening". United States. https://www.osti.gov/servlets/purl/968305.
@article{osti_968305,
title = {High throughput protein production screening},
author = {Beernink, Peter T and Coleman, Matthew A and Segelke, Brent W},
abstractNote = {Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {9}
}

Patent:

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