High throughput protein production screening

Beernink, Peter T; Coleman, Matthew A; Segelke, Brent W

High throughput protein production screening

Patent · 08 September 2009

OSTI ID:968305

Beernink, Peter T ^[1]; Coleman, Matthew A ^[2]; Segelke, Brent W ^[3]

Walnut Creek, CA
Oakland, CA
San Ramon, CA

Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA

Sponsoring Organization:: United States Department of Energy

DOE Contract Number:: W-7405-ENG-48

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Number(s):: 7,585,815

Application Number:: 10/884,783

OSTI ID:: 968305

Country of Publication:: United States

Language:: English

References (18)

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A Human cDNA Library for High-Throughput Protein Expression Screening Büssow, Konrad; Nordhoff, Eckhard; Lübbert, Christine Genomics, Vol. 65, Issue 1 https://doi.org/10.1006/geno.2000.6141	journal	April 2000
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High-Throughput, Fluorescence-Based Screening for Soluble Protein Expression Coleman, M. A.; Lao, V. H.; Segelke, B. W. Journal of Proteome Research, Vol. 3, Issue 5 https://doi.org/10.1021/pr049912g	journal	October 2004
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