Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes
Abstract
The use of Layer-by-layer techniques for immobilizing several types of enzymes, e.g. glucose oxidase (GOx), horse radish oxidases(HRP), and choline oxidase(CHO) on carbon nanotubes and their applications for biosenseing are presented. The enzyme is immobilized on the negatively charged CNT surface by alternatively assembling a cationic polydiallyldimethyl-ammonium chloride (PDDA) layer and a enzyme layer. The sandwich-like layer structure (PDDA/enzyme/PDDA/CNT) formed by electrostatic assembling provides a favorable microenvironment to keep the bioactivity of enzyme and to prevent enzyme molecule leakage. The morphologies and electrocatalytic acitivity of the resulted enzyme film were characterized using TEM and electrochemical techniques, respectively. It was found that these enzyme-based biosensors are very sensitive, selective for detection of biomolecules, e.g. glucose, choline.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 966307
- Report Number(s):
- PNNL-SA-53316
10302; 24822; TRN: US200921%%260
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Book
- Resource Relation:
- Related Information: Biomolecular Catalysis: Nanoscale Science and Technology, ACS Symposium Series 986:117-128
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CARBON; IMMOBILIZED ENZYMES; FABRICATION; NANOTUBES; OXIDASES; ENZYME ACTIVITY; MORPHOLOGY; ELECTROCATALYSTS; GLUCOSE; CHOLINE; DETECTION; carbon nanotubes; biosensors; Environmental Molecular Sciences Laboratory
Citation Formats
Wang, Jun, Liu, Guodong, and Lin, Yuehe. Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes. United States: N. p., 2008.
Web.
Wang, Jun, Liu, Guodong, & Lin, Yuehe. Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes. United States.
Wang, Jun, Liu, Guodong, and Lin, Yuehe. 2008.
"Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes". United States.
@article{osti_966307,
title = {Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes},
author = {Wang, Jun and Liu, Guodong and Lin, Yuehe},
abstractNote = {The use of Layer-by-layer techniques for immobilizing several types of enzymes, e.g. glucose oxidase (GOx), horse radish oxidases(HRP), and choline oxidase(CHO) on carbon nanotubes and their applications for biosenseing are presented. The enzyme is immobilized on the negatively charged CNT surface by alternatively assembling a cationic polydiallyldimethyl-ammonium chloride (PDDA) layer and a enzyme layer. The sandwich-like layer structure (PDDA/enzyme/PDDA/CNT) formed by electrostatic assembling provides a favorable microenvironment to keep the bioactivity of enzyme and to prevent enzyme molecule leakage. The morphologies and electrocatalytic acitivity of the resulted enzyme film were characterized using TEM and electrochemical techniques, respectively. It was found that these enzyme-based biosensors are very sensitive, selective for detection of biomolecules, e.g. glucose, choline.},
doi = {},
url = {https://www.osti.gov/biblio/966307},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jun 01 00:00:00 EDT 2008},
month = {Sun Jun 01 00:00:00 EDT 2008}
}