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Title: Quantitation of fungal mRNAs in complex substrates by reverse transciption pcr and its application to Phanerochaete chrysosporium-colonized soil

Abstract

Thorough analysis of fungi in complex substrates has been hampered by inadequate experimental tools for assessing physiological activity and estimating biomass. We report a method for the quantitative assessment of specific fungal mRNAs in soil. The method was applied to complex gene families of Phanerochaete chrysosporium, a white-rot fungus widely used in studies of organopollutant degradation. Among the genes implicated in pollutant degradation, two closely related lignin peroxidase transcripts were detected in soil. The pattern of lignin peroxidase gene expression was unexpected; certain transcripts abundant in defined cultures were not detected in soil cultures. Transcripts encoding cellobiohydrolases and{beta}-tubulin were also detected. The method will aid in defining the roles of specific genes in complex biological processes such as organopollutant degradation, developing strategies for strain improvement, and identifying specific fungi in environmental samples. 45 refs., 3 figs., 1 tab.

Authors:
; ;  [1]
  1. Univ. of Wisconsin, Madison, WI (United States) [and others
Publication Date:
OSTI Identifier:
96608
DOE Contract Number:  
FG02-87ER13712
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 61; Journal Issue: 6; Other Information: PBD: Jun 1995
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; FUNGI; CLASSIFICATION; MESSENGER-RNA; ECOLOGICAL CONCENTRATION; SOILS; QUANTITATIVE CHEMICAL ANALYSIS; CONTAMINATION; POLLUTANTS; BIODEGRADATION; PHANEROCHAETE

Citation Formats

Lamar, R.T., Schoenike, B., and Dietrich, D.M. Quantitation of fungal mRNAs in complex substrates by reverse transciption pcr and its application to Phanerochaete chrysosporium-colonized soil. United States: N. p., 1995. Web.
Lamar, R.T., Schoenike, B., & Dietrich, D.M. Quantitation of fungal mRNAs in complex substrates by reverse transciption pcr and its application to Phanerochaete chrysosporium-colonized soil. United States.
Lamar, R.T., Schoenike, B., and Dietrich, D.M. Thu . "Quantitation of fungal mRNAs in complex substrates by reverse transciption pcr and its application to Phanerochaete chrysosporium-colonized soil". United States.
@article{osti_96608,
title = {Quantitation of fungal mRNAs in complex substrates by reverse transciption pcr and its application to Phanerochaete chrysosporium-colonized soil},
author = {Lamar, R.T. and Schoenike, B. and Dietrich, D.M.},
abstractNote = {Thorough analysis of fungi in complex substrates has been hampered by inadequate experimental tools for assessing physiological activity and estimating biomass. We report a method for the quantitative assessment of specific fungal mRNAs in soil. The method was applied to complex gene families of Phanerochaete chrysosporium, a white-rot fungus widely used in studies of organopollutant degradation. Among the genes implicated in pollutant degradation, two closely related lignin peroxidase transcripts were detected in soil. The pattern of lignin peroxidase gene expression was unexpected; certain transcripts abundant in defined cultures were not detected in soil cultures. Transcripts encoding cellobiohydrolases and{beta}-tubulin were also detected. The method will aid in defining the roles of specific genes in complex biological processes such as organopollutant degradation, developing strategies for strain improvement, and identifying specific fungi in environmental samples. 45 refs., 3 figs., 1 tab.},
doi = {},
journal = {Applied and Environmental Microbiology},
number = 6,
volume = 61,
place = {United States},
year = {1995},
month = {6}
}