A negative element involved in Kaposi's sarcoma-associated herpesvirus-encoded ORF11 gene expression

Journal Article · Thu Jan 01 00:00:00 EST 2009 · Journal of Virology

OSTI ID:962301

Chen, Lei ^[1]

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1. Los Alamos National Laboratory

The ORF11 of the Kaposi's sarcoma-associated herpesvirus (KSHV) is a lytic viral gene with delayed-early expression kinetics. How the ORF11 gene expression is regulated in the KSHV lytic cascade is largely unknown. Here we report that the deletion of the KSHV viral IL-6 gene from the viral genome leads to deregulated ORF11 gene expression. The KSHV-encoded viral IL-6 protein was found not to be essentially involved in the regulation of ORF11, suggesting a potential transcriptional cis-regulation. A negative element was identified downstream of the ORF11 gene, which suppresses the ORF11 basal promoter activity in a position-independent manner.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC52-06NA25396
• OSTI ID: 962301
• Report Number(s): LA-UR-09-01304; LA-UR-09-1304; JOVIAM; TRN: US200919%%64
• Journal Information: Journal of Virology, Journal Name: Journal of Virology; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
KINETICS
PROMOTERS
PROTEINS
REGULATIONS