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Title: Expression, Purification and Crystallization of Human 5-Lipoxygeanse-Activating Protein with Leukotriene-Biosynthesis Inhibitors

Abstract

The nuclear membrane protein 5-lipoxygenase-activating protein (FLAP) plays an essential role in leukotriene synthesis. Recombinant full-length human FLAP with a C-terminal hexahistidine tag has been expressed and purified from the cytoplasmic membrane of Escherichia coli. Diffraction-quality crystals of FLAP in complex with leukotriene-synthesis inhibitor MK-591 and with an iodinated analogue of MK-591 have been grown using the sitting-drop vapor-diffusion method. The crystals exhibit tetragonal symmetry (P4212) and diffracted to a resolution limit of 4 Angstroms .

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
960092
Report Number(s):
BNL-83078-2009-JA
TRN: US201016%%1236
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica Section F: Structural Biology and Crystallization Communications; Journal Volume: 63
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CRYSTALLIZATION; ESCHERICHIA COLI; MEMBRANE PROTEINS; MEMBRANES; PROTEINS; PURIFICATION; RESOLUTION; SYMMETRY; SYNTHESIS; CRYSTALS; national synchrotron light source

Citation Formats

Xu,S., McKeever, B., Wisniewski, D., Miller, D., Spencer, R., Chu, L., Ujjainwalla, F., Yamin, T., Evans, J., and et al. Expression, Purification and Crystallization of Human 5-Lipoxygeanse-Activating Protein with Leukotriene-Biosynthesis Inhibitors. United States: N. p., 2007. Web. doi:10.1107/S1744309107055571.
Xu,S., McKeever, B., Wisniewski, D., Miller, D., Spencer, R., Chu, L., Ujjainwalla, F., Yamin, T., Evans, J., & et al. Expression, Purification and Crystallization of Human 5-Lipoxygeanse-Activating Protein with Leukotriene-Biosynthesis Inhibitors. United States. doi:10.1107/S1744309107055571.
Xu,S., McKeever, B., Wisniewski, D., Miller, D., Spencer, R., Chu, L., Ujjainwalla, F., Yamin, T., Evans, J., and et al. Mon . "Expression, Purification and Crystallization of Human 5-Lipoxygeanse-Activating Protein with Leukotriene-Biosynthesis Inhibitors". United States. doi:10.1107/S1744309107055571.
@article{osti_960092,
title = {Expression, Purification and Crystallization of Human 5-Lipoxygeanse-Activating Protein with Leukotriene-Biosynthesis Inhibitors},
author = {Xu,S. and McKeever, B. and Wisniewski, D. and Miller, D. and Spencer, R. and Chu, L. and Ujjainwalla, F. and Yamin, T. and Evans, J. and et al},
abstractNote = {The nuclear membrane protein 5-lipoxygenase-activating protein (FLAP) plays an essential role in leukotriene synthesis. Recombinant full-length human FLAP with a C-terminal hexahistidine tag has been expressed and purified from the cytoplasmic membrane of Escherichia coli. Diffraction-quality crystals of FLAP in complex with leukotriene-synthesis inhibitor MK-591 and with an iodinated analogue of MK-591 have been grown using the sitting-drop vapor-diffusion method. The crystals exhibit tetragonal symmetry (P4212) and diffracted to a resolution limit of 4 Angstroms .},
doi = {10.1107/S1744309107055571},
journal = {Acta Crystallographica Section F: Structural Biology and Crystallization Communications},
number = ,
volume = 63,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}