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Title: Structural Metals in the Group I Intron: A Ribozyme with a Multiple Metal Ion Core

Abstract

Metal ions play key roles in the folding and function for many structured RNAs, including group I introns. We determined the X-ray crystal structure of the Azoarcus bacterial group I intron in complex with its 5' and 3' exons. In addition to 222 nucleotides of RNA, the model includes 18 Mg2+ and K+ ions. Five of the metals bind within 12 Angstroms of the scissile phosphate and coordinate the majority of the oxygen atoms biochemically implicated in conserved metal-RNA interactions. The metals are buried deep within the structure and form a multiple metal ion core that is critical to group I intron structure and function. Eight metal ions bind in other conserved regions of the intron structure, and the remaining five interact with peripheral structural elements. Each of the 18 metals mediates tertiary interactions, facilitates local bends in the sugar-phosphate backbone or binds in the major groove of helices. The group I intron has a rich history of biochemical efforts aimed to identify RNA-metal ion interactions. The structural data are correlated to the biochemical results to further understand the role of metal ions in group I intron structure and function.

Authors:
; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
959741
Report Number(s):
BNL-82727-2009-JA
Journal ID: ISSN 0022-2836; JMOBAK; TRN: US201016%%885
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Molecular Biology; Journal Volume: 372; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; ATOMS; CRYSTAL STRUCTURE; EXONS; INTRONS; NUCLEOTIDES; OXYGEN; PHOSPHATES; RNA; national synchrotron light source

Citation Formats

Stahley,M., Adams, P., Wang, J., and Strobel, S.. Structural Metals in the Group I Intron: A Ribozyme with a Multiple Metal Ion Core. United States: N. p., 2007. Web. doi:10.1016/j.jmb.2007.06.026.
Stahley,M., Adams, P., Wang, J., & Strobel, S.. Structural Metals in the Group I Intron: A Ribozyme with a Multiple Metal Ion Core. United States. doi:10.1016/j.jmb.2007.06.026.
Stahley,M., Adams, P., Wang, J., and Strobel, S.. Mon . "Structural Metals in the Group I Intron: A Ribozyme with a Multiple Metal Ion Core". United States. doi:10.1016/j.jmb.2007.06.026.
@article{osti_959741,
title = {Structural Metals in the Group I Intron: A Ribozyme with a Multiple Metal Ion Core},
author = {Stahley,M. and Adams, P. and Wang, J. and Strobel, S.},
abstractNote = {Metal ions play key roles in the folding and function for many structured RNAs, including group I introns. We determined the X-ray crystal structure of the Azoarcus bacterial group I intron in complex with its 5' and 3' exons. In addition to 222 nucleotides of RNA, the model includes 18 Mg2+ and K+ ions. Five of the metals bind within 12 Angstroms of the scissile phosphate and coordinate the majority of the oxygen atoms biochemically implicated in conserved metal-RNA interactions. The metals are buried deep within the structure and form a multiple metal ion core that is critical to group I intron structure and function. Eight metal ions bind in other conserved regions of the intron structure, and the remaining five interact with peripheral structural elements. Each of the 18 metals mediates tertiary interactions, facilitates local bends in the sugar-phosphate backbone or binds in the major groove of helices. The group I intron has a rich history of biochemical efforts aimed to identify RNA-metal ion interactions. The structural data are correlated to the biochemical results to further understand the role of metal ions in group I intron structure and function.},
doi = {10.1016/j.jmb.2007.06.026},
journal = {Journal of Molecular Biology},
number = 1,
volume = 372,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}