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Title: Architecture of a Coat for the Nuclear Pore Membrane

Abstract

The symmetric core of the nuclear pore complex can be considered schematically as a series of concentric cylinders. A peripheral cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a hetero-octamer in two space groups. Oligomerization of Sec13-Nup145C was confirmed biochemically. Importantly, the numerous interacting surfaces in the hetero-octamer are evolutionarily highly conserved, further underlining the physiological relevance of the oligomerization. The hetero-octamer forms a slightly curved, yet rigid rod of sufficient length to span the entire height of the proposed membrane-adjacent cylinder. In concordance with the dimensions and symmetry of the nuclear pore complex core, we suggest that the cylinder is constructed of four antiparallel rings, each ring being composed of eight heptamers arranged in a head-to-tail fashion. Our model proposes that the hetero-octamer would vertically traverse and connect the four stacked rings.

Authors:
; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
959563
Report Number(s):
BNL-82549-2009-JA
Journal ID: ISSN 0092-8674; CELLB5; TRN: US201016%%707
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cell; Journal Volume: 131; Journal Issue: 7
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ARCHITECTURE; COATINGS; DIMENSIONS; MEMBRANES; SPACE GROUPS; SYMMETRY; national synchrotron light source

Citation Formats

Hsia,K., Stavropoulos, P., Blobel, G., and Hoelz, A. Architecture of a Coat for the Nuclear Pore Membrane. United States: N. p., 2007. Web. doi:10.1016/j.cell.2007.11.038.
Hsia,K., Stavropoulos, P., Blobel, G., & Hoelz, A. Architecture of a Coat for the Nuclear Pore Membrane. United States. doi:10.1016/j.cell.2007.11.038.
Hsia,K., Stavropoulos, P., Blobel, G., and Hoelz, A. Mon . "Architecture of a Coat for the Nuclear Pore Membrane". United States. doi:10.1016/j.cell.2007.11.038.
@article{osti_959563,
title = {Architecture of a Coat for the Nuclear Pore Membrane},
author = {Hsia,K. and Stavropoulos, P. and Blobel, G. and Hoelz, A.},
abstractNote = {The symmetric core of the nuclear pore complex can be considered schematically as a series of concentric cylinders. A peripheral cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a hetero-octamer in two space groups. Oligomerization of Sec13-Nup145C was confirmed biochemically. Importantly, the numerous interacting surfaces in the hetero-octamer are evolutionarily highly conserved, further underlining the physiological relevance of the oligomerization. The hetero-octamer forms a slightly curved, yet rigid rod of sufficient length to span the entire height of the proposed membrane-adjacent cylinder. In concordance with the dimensions and symmetry of the nuclear pore complex core, we suggest that the cylinder is constructed of four antiparallel rings, each ring being composed of eight heptamers arranged in a head-to-tail fashion. Our model proposes that the hetero-octamer would vertically traverse and connect the four stacked rings.},
doi = {10.1016/j.cell.2007.11.038},
journal = {Cell},
number = 7,
volume = 131,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}