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Title: Asymmetri Distribution of Metals in the Xenopus Laevis Oocyte: a Synchrotron X-Ray Fluorescence Microprobe Study

Abstract

The asymmetric distribution of many components of the Xenopus oocyte, including RNA, proteins, and pigment, provides a framework for cellular specialization during development. During maturation, Xenopus oocytes also acquire metals needed for development, but apart from zinc, little is known about their distribution. Synchrotron X-ray fluorescence microprobe was used to map iron, copper, and zinc and the metalloid selenium in a whole oocyte. Iron, zinc, and copper were asymmetrically distributed in the cytoplasm, while selenium and copper were more abundant in the nucleus. A zone of high copper and zinc was seen in the animal pole cytoplasm. Iron was also concentrated in the animal pole but did not colocalize with zinc, copper, or pigment accumulations. This asymmetry of metal deposition may be important for normal development. Synchrotron X-ray fluorescence microprobe will be a useful tool to examine how metals accumulate and redistribute during fertilization and embryonic development.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Stanford Linear Accelerator Center (SLAC)
Sponsoring Org.:
USDOE
OSTI Identifier:
958668
Report Number(s):
SLAC-REPRINT-2009-004
TRN: US1000303
DOE Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article
Journal Name:
Biochem.Cell Biol.85:537,2007
Additional Journal Information:
Journal Volume: 85; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; ASYMMETRY; COPPER; CYTOPLASM; DEPOSITION; DISTRIBUTION; FERTILIZATION; FLUORESCENCE; IRON; ONTOGENESIS; OOCYTES; PIGMENTS; PROTEINS; RNA; SELENIUM; SEMIMETALS; SYNCHROTRONS; ZINC; BIO, CHEM

Citation Formats

Popescu, B.F.G., Belak, Z.R., Ignatyev, K., Ovsenek, N., Nichol, H., and /Saskatchewan U. /SLAC, SSRL. Asymmetri Distribution of Metals in the Xenopus Laevis Oocyte: a Synchrotron X-Ray Fluorescence Microprobe Study. United States: N. p., 2009. Web.
Popescu, B.F.G., Belak, Z.R., Ignatyev, K., Ovsenek, N., Nichol, H., & /Saskatchewan U. /SLAC, SSRL. Asymmetri Distribution of Metals in the Xenopus Laevis Oocyte: a Synchrotron X-Ray Fluorescence Microprobe Study. United States.
Popescu, B.F.G., Belak, Z.R., Ignatyev, K., Ovsenek, N., Nichol, H., and /Saskatchewan U. /SLAC, SSRL. Wed . "Asymmetri Distribution of Metals in the Xenopus Laevis Oocyte: a Synchrotron X-Ray Fluorescence Microprobe Study". United States.
@article{osti_958668,
title = {Asymmetri Distribution of Metals in the Xenopus Laevis Oocyte: a Synchrotron X-Ray Fluorescence Microprobe Study},
author = {Popescu, B.F.G. and Belak, Z.R. and Ignatyev, K. and Ovsenek, N. and Nichol, H. and /Saskatchewan U. /SLAC, SSRL},
abstractNote = {The asymmetric distribution of many components of the Xenopus oocyte, including RNA, proteins, and pigment, provides a framework for cellular specialization during development. During maturation, Xenopus oocytes also acquire metals needed for development, but apart from zinc, little is known about their distribution. Synchrotron X-ray fluorescence microprobe was used to map iron, copper, and zinc and the metalloid selenium in a whole oocyte. Iron, zinc, and copper were asymmetrically distributed in the cytoplasm, while selenium and copper were more abundant in the nucleus. A zone of high copper and zinc was seen in the animal pole cytoplasm. Iron was also concentrated in the animal pole but did not colocalize with zinc, copper, or pigment accumulations. This asymmetry of metal deposition may be important for normal development. Synchrotron X-ray fluorescence microprobe will be a useful tool to examine how metals accumulate and redistribute during fertilization and embryonic development.},
doi = {},
journal = {Biochem.Cell Biol.85:537,2007},
number = 5,
volume = 85,
place = {United States},
year = {2009},
month = {4}
}