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Title: Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells

Abstract

Healthy human males produce sperm cells of which about 25-40% have abnormal head shapes. Increases in the percentage of sperm exhibiting aberrant sperm head morphologies have been correlated with male infertility, and biochemical studies of pooled sperm have suggested that sperm with abnormal shape may contain DNA that has not been properly repackaged by protamine during spermatid development. We have used micro-Raman spectroscopy to obtain Raman spectra from individual human sperm cells and examined how differences in the Raman spectra of sperm chromatin correlate with cell shape. We show that Raman spectra of individual sperm cells contain vibrational marker modes that can be used to assess the efficiency of DNA-packaging for each cell. Raman spectra obtained from sperm cells with normal shape provide evidence that DNA in these sperm is very efficiently packaged. We find, however, that the relative protein content per cell and DNA packaging efficiencies are distributed over a relatively wide range for sperm cells with both normal and abnormal shape. These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in-vitro fertilization.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
957626
Report Number(s):
LLNL-JRNL-411195
TRN: US201016%%68
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Biophotonics, vol. 2, no. 5, May 1, 2009, pp. 322-332; Journal Volume: 2; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMATIN; DNA; EFFICIENCY; FERTILIZATION; IN VITRO; MALES; PACKAGING; PROTAMINES; PROTEINS; RAMAN SPECTRA; RAMAN SPECTROSCOPY; SHAPE; SPECTROSCOPY; SPERMATOZOA

Citation Formats

Huser, T, Orme, C, Hollars, C, Corzett, M, and Balhorn, R. Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells. United States: N. p., 2009. Web. doi:10.1002/jbio.200910012.
Huser, T, Orme, C, Hollars, C, Corzett, M, & Balhorn, R. Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells. United States. doi:10.1002/jbio.200910012.
Huser, T, Orme, C, Hollars, C, Corzett, M, and Balhorn, R. Mon . "Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells". United States. doi:10.1002/jbio.200910012. https://www.osti.gov/servlets/purl/957626.
@article{osti_957626,
title = {Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells},
author = {Huser, T and Orme, C and Hollars, C and Corzett, M and Balhorn, R},
abstractNote = {Healthy human males produce sperm cells of which about 25-40% have abnormal head shapes. Increases in the percentage of sperm exhibiting aberrant sperm head morphologies have been correlated with male infertility, and biochemical studies of pooled sperm have suggested that sperm with abnormal shape may contain DNA that has not been properly repackaged by protamine during spermatid development. We have used micro-Raman spectroscopy to obtain Raman spectra from individual human sperm cells and examined how differences in the Raman spectra of sperm chromatin correlate with cell shape. We show that Raman spectra of individual sperm cells contain vibrational marker modes that can be used to assess the efficiency of DNA-packaging for each cell. Raman spectra obtained from sperm cells with normal shape provide evidence that DNA in these sperm is very efficiently packaged. We find, however, that the relative protein content per cell and DNA packaging efficiencies are distributed over a relatively wide range for sperm cells with both normal and abnormal shape. These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in-vitro fertilization.},
doi = {10.1002/jbio.200910012},
journal = {Journal of Biophotonics, vol. 2, no. 5, May 1, 2009, pp. 322-332},
number = 5,
volume = 2,
place = {United States},
year = {Mon Mar 09 00:00:00 EDT 2009},
month = {Mon Mar 09 00:00:00 EDT 2009}
}