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Title: Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Abstract

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

Inventors:
 [1];  [2];  [3]
  1. Richland, WA
  2. Fall City, WA
  3. Pasco, WA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
956997
Patent Number(s):
7,449,569
Application Number:
10/920,625
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
DE-AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States: N. p., 2008. Web.
Dai, Ziyu, Lasure, Linda L, & Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States.
Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. 2008. "Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus". United States. https://www.osti.gov/servlets/purl/956997.
@article{osti_956997,
title = {Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus},
author = {Dai, Ziyu and Lasure, Linda L and Magnuson, Jon K},
abstractNote = {The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.},
doi = {},
url = {https://www.osti.gov/biblio/956997}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {11}
}

Works referenced in this record:

Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays
journal, August 1987


GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
journal, December 1987


Identification of Genes Associated with Morphology in Aspergillus niger by Using Suppression Subtractive Hybridization
journal, April 2004


Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi
journal, March 1989