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Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Patent ·
OSTI ID:956997
 [1];  [2];  [3]
  1. Richland, WA
  2. Fall City, WA
  3. Pasco, WA
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The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
7,449,569
Application Number:
10/920,625
OSTI ID:
956997
Country of Publication:
United States
Language:
English

References (5)

GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. journal December 1987
Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi journal March 1989
Growth Media book January 1991
Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays journal August 1987
Identification of Genes Associated with Morphology in Aspergillus niger by Using Suppression Subtractive Hybridization journal April 2004

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