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Title: New Paradigm for Macromolecular Crystallography Experiments at SSRL: Automated Crystal Screening And Remote Data Collection

Abstract

Complete automation of the macromolecular crystallography experiment has been achieved at Stanford Synchrotron Radiation Lightsource (SSRL) through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface. These highly reliable systems have enabled crystallography experiments to be carried out from the researchers' home institutions and other remote locations while retaining complete control over even the most challenging systems. A breakthrough component of the system, the Stanford Auto-Mounter (SAM), has enabled the efficient mounting of cryocooled samples without human intervention. Taking advantage of this automation, researchers have successfully screened more than 200 000 samples to select the crystals with the best diffraction quality for data collection as well as to determine optimal crystallization and cryocooling conditions. These systems, which have been deployed on all SSRL macromolecular crystallography beamlines and several beamlines worldwide, are used by more than 80 research groups in remote locations, establishing a new paradigm for macromolecular crystallography experimentation.

Authors:
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Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
953572
Report Number(s):
SLAC-REPRINT-2009-312
Journal ID: ISSN 0907-4449; TRN: US1000848
DOE Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article
Journal Name:
Acta Crystallogr.D Biol.Crystallogr.D64:1210,2008
Additional Journal Information:
Journal Volume: 64; Journal Issue: Pt12; Journal ID: ISSN 0907-4449
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; AUTOMATION; CONTROL; CONTROL SYSTEMS; CRYSTALLIZATION; CRYSTALLOGRAPHY; CRYSTALS; DATA; DIFFRACTION; LIGHT SOURCES; STANFORD LINEAR ACCELERATOR CENTER; SYNCHROTRON RADIATION; Other,OTHER, BIO

Citation Formats

Soltis, S M, Cohen, A E, Deacon, A, Eriksson, T, Gonzalez, A, McPhillips, S, Chui, H, Dunten, P, Hollenbeck, M, Mathews, I, Miller, M, Moorhead, P, Phizackerley, R P, Smith, C, Song, J, Bedem, H van dem, Ellis, P, Kuhn, P, McPhillips, T, Sauter, N, and Sharp, K. New Paradigm for Macromolecular Crystallography Experiments at SSRL: Automated Crystal Screening And Remote Data Collection. United States: N. p., 2009. Web.
Soltis, S M, Cohen, A E, Deacon, A, Eriksson, T, Gonzalez, A, McPhillips, S, Chui, H, Dunten, P, Hollenbeck, M, Mathews, I, Miller, M, Moorhead, P, Phizackerley, R P, Smith, C, Song, J, Bedem, H van dem, Ellis, P, Kuhn, P, McPhillips, T, Sauter, N, & Sharp, K. New Paradigm for Macromolecular Crystallography Experiments at SSRL: Automated Crystal Screening And Remote Data Collection. United States.
Soltis, S M, Cohen, A E, Deacon, A, Eriksson, T, Gonzalez, A, McPhillips, S, Chui, H, Dunten, P, Hollenbeck, M, Mathews, I, Miller, M, Moorhead, P, Phizackerley, R P, Smith, C, Song, J, Bedem, H van dem, Ellis, P, Kuhn, P, McPhillips, T, Sauter, N, and Sharp, K. 2009. "New Paradigm for Macromolecular Crystallography Experiments at SSRL: Automated Crystal Screening And Remote Data Collection". United States.
@article{osti_953572,
title = {New Paradigm for Macromolecular Crystallography Experiments at SSRL: Automated Crystal Screening And Remote Data Collection},
author = {Soltis, S M and Cohen, A E and Deacon, A and Eriksson, T and Gonzalez, A and McPhillips, S and Chui, H and Dunten, P and Hollenbeck, M and Mathews, I and Miller, M and Moorhead, P and Phizackerley, R P and Smith, C and Song, J and Bedem, H van dem and Ellis, P and Kuhn, P and McPhillips, T and Sauter, N and Sharp, K},
abstractNote = {Complete automation of the macromolecular crystallography experiment has been achieved at Stanford Synchrotron Radiation Lightsource (SSRL) through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface. These highly reliable systems have enabled crystallography experiments to be carried out from the researchers' home institutions and other remote locations while retaining complete control over even the most challenging systems. A breakthrough component of the system, the Stanford Auto-Mounter (SAM), has enabled the efficient mounting of cryocooled samples without human intervention. Taking advantage of this automation, researchers have successfully screened more than 200 000 samples to select the crystals with the best diffraction quality for data collection as well as to determine optimal crystallization and cryocooling conditions. These systems, which have been deployed on all SSRL macromolecular crystallography beamlines and several beamlines worldwide, are used by more than 80 research groups in remote locations, establishing a new paradigm for macromolecular crystallography experimentation.},
doi = {},
url = {https://www.osti.gov/biblio/953572}, journal = {Acta Crystallogr.D Biol.Crystallogr.D64:1210,2008},
issn = {0907-4449},
number = Pt12,
volume = 64,
place = {United States},
year = {2009},
month = {5}
}