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Title: New Catalytic DNA Biosensors for Radionuclides and Metal ion

Abstract

We aim to develop new DNA biosensors for simultaneous detection and quantification of bioavailable radionuclides, such as uranium, technetium, and plutonium, and metal contaminants, such as lead, chromium, and mercury. The sensors will be highly sensitive and selective. They will be applied to on-site, real-time assessment of concentration, speciation, and stability of the individual contaminants before and during bioremediation, and for long-term monitoring of DOE contaminated sites. To achieve this goal, we have employed a combinatorial method called “in vitro selection” to search from a large DNA library (~ 1015 different molecules) for catalytic DNA molecules that are highly specific for radionuclides or other metal ions through intricate 3-dimensional interactions as in metalloproteins. Comprehensive biochemical and biophysical studies have been performed on the selected DNA molecules. The findings from these studies have helped to elucidate fundamental principles for designing effective sensors for radionuclides and metal ions. Based on the study, the DNA have been converted to fluorescent or colorimetric sensors by attaching to it fluorescent donor/acceptor pairs or gold nanoparticles, with 11 part-per-trillion detection limit (for uranium) and over million fold selectivity (over other radionuclides and metal ions tested). Practical application of the biosensors for samples from the Environmental Remediationmore » Sciences Program (ERSP) Field Research Center (FRC) at Oak Ridge has also been demonstrated.« less

Authors:
Publication Date:
Research Org.:
University of Illinois at Urbana-Champaign
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
951056
Report Number(s):
DOE/ER/63179-1
TRN: US201003%%698
DOE Contract Number:  
FG02-01ER63179
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIOREMEDIATION; CHROMIUM; DETECTION; DNA; GOLD; MERCURY; METALLOPROTEINS; MONITORING; PLUTONIUM; RADIOISOTOPES; SENSITIVITY; STABILITY; TECHNETIUM; URANIUM; Sensor, Radionuclides, Metal ions, Remediation, monitor, on-site detection

Citation Formats

Yi Lu. New Catalytic DNA Biosensors for Radionuclides and Metal ion. United States: N. p., 2008. Web. doi:10.2172/951056.
Yi Lu. New Catalytic DNA Biosensors for Radionuclides and Metal ion. United States. doi:10.2172/951056.
Yi Lu. Sat . "New Catalytic DNA Biosensors for Radionuclides and Metal ion". United States. doi:10.2172/951056. https://www.osti.gov/servlets/purl/951056.
@article{osti_951056,
title = {New Catalytic DNA Biosensors for Radionuclides and Metal ion},
author = {Yi Lu},
abstractNote = {We aim to develop new DNA biosensors for simultaneous detection and quantification of bioavailable radionuclides, such as uranium, technetium, and plutonium, and metal contaminants, such as lead, chromium, and mercury. The sensors will be highly sensitive and selective. They will be applied to on-site, real-time assessment of concentration, speciation, and stability of the individual contaminants before and during bioremediation, and for long-term monitoring of DOE contaminated sites. To achieve this goal, we have employed a combinatorial method called “in vitro selection” to search from a large DNA library (~ 1015 different molecules) for catalytic DNA molecules that are highly specific for radionuclides or other metal ions through intricate 3-dimensional interactions as in metalloproteins. Comprehensive biochemical and biophysical studies have been performed on the selected DNA molecules. The findings from these studies have helped to elucidate fundamental principles for designing effective sensors for radionuclides and metal ions. Based on the study, the DNA have been converted to fluorescent or colorimetric sensors by attaching to it fluorescent donor/acceptor pairs or gold nanoparticles, with 11 part-per-trillion detection limit (for uranium) and over million fold selectivity (over other radionuclides and metal ions tested). Practical application of the biosensors for samples from the Environmental Remediation Sciences Program (ERSP) Field Research Center (FRC) at Oak Ridge has also been demonstrated.},
doi = {10.2172/951056},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 2008},
month = {Sat Mar 01 00:00:00 EST 2008}
}

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