skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: X-ray absorption fine-structure determination of pH-dependent U-bacterial cell wall interactions.

Abstract

X-ray absorption fine structure (XAFS) measurements was used at the U L3-edge to directly determine the pH dependence of the cell wall functional groups responsible for the absorption of aqueous UO{sub 2}{sup 2+} to Bacillus subtilis from pH 1.67 to 4.80. Surface complexation modeling can be used to predict metal distributions in water-rock systems, and it has been used to quantify bacterial adsorption of metal cations. However, successful application of these models requires a detailed knowledge not only of the type of bacterial surface site involved in metal adsorption/desorption, but also of the binding geometry. Previous acid-base titrations of B. subtilis cells suggested that three surface functional group types are important on the cell wall; these groups have been postulated to correspond to carboxyl, phosphoryl, and hydroxyl sites. When the U(VI) adsorption to B. subtilis is measured, observed is a significant pH-independent absorption at low pH values (<3.0), ascribed to an interaction between the uranyl cation and a neutrally charged phosphoryl group on the cell wall. The present study provides independent quantitative constraints on the types of sites involved in uranyl binding to B. subtilis from pH 1.67 to 4.80. The XAFS results indicate that at extremely low pH (pHmore » 1.67) UO{sub 2}{sup 2+} binds exclusively to phosphoryl functional groups on the cell wall, with an average distance between the U atom and the P atom of 3.64 {+-} 0.01 Angstroms. This U-P distance indicates an inner-sphere complex with an oxygen atom shared between the UO{sub 2}{sup 2+} and the phosphoryl ligand. The P signal at extremely low pH value is consistent with the UO{sub 2}{sup 2+} binding to a protonated phosphoryl group, as previously ascribed. With increasing pH (3.22 and 4.80), UO{sub 2}{sup 2+} binds increasingly to bacterial surface carboxyl functional groups, with an average distance between the U atom and the C atom of 2.89 {+-} 0.02 Angstroms. This U-C distance indicates an inner-sphere complex with two oxygen atoms shared between the UO{sub 2}{sup 2+} and the carboxyl ligand. The results of this XAFS study confirm the uranyl-bacterial surface speciation model.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Science Foundation (NSF); OUS
OSTI Identifier:
949419
Report Number(s):
ANL/ER/JA-40750
Journal ID: ISSN 0016-7037; GCACAK; TRN: US201012%%214
DOE Contract Number:  
DE-AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Geochim. Cosmochim. Acta
Additional Journal Information:
Journal Volume: 66; Journal Issue: 22 ; 2002; Journal ID: ISSN 0016-7037
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ABSORPTION; ADSORPTION; ATOMS; BACILLUS SUBTILIS; CATIONS; CELL WALL; FINE STRUCTURE; FUNCTIONALS; GEOMETRY; OXYGEN; PH VALUE; SIMULATION; X-RAY SPECTROSCOPY

Citation Formats

Kelly, S D, Kemner, K M, Fein, J B, Fowle, D A, Boyanov, M I, Bunker, B A, Yee, N, Environmental Research, and Univ. of Notre Dame. X-ray absorption fine-structure determination of pH-dependent U-bacterial cell wall interactions.. United States: N. p., 2002. Web. doi:10.1016/S0016-7037(02)00947-X.
Kelly, S D, Kemner, K M, Fein, J B, Fowle, D A, Boyanov, M I, Bunker, B A, Yee, N, Environmental Research, & Univ. of Notre Dame. X-ray absorption fine-structure determination of pH-dependent U-bacterial cell wall interactions.. United States. doi:10.1016/S0016-7037(02)00947-X.
Kelly, S D, Kemner, K M, Fein, J B, Fowle, D A, Boyanov, M I, Bunker, B A, Yee, N, Environmental Research, and Univ. of Notre Dame. Tue . "X-ray absorption fine-structure determination of pH-dependent U-bacterial cell wall interactions.". United States. doi:10.1016/S0016-7037(02)00947-X.
@article{osti_949419,
title = {X-ray absorption fine-structure determination of pH-dependent U-bacterial cell wall interactions.},
author = {Kelly, S D and Kemner, K M and Fein, J B and Fowle, D A and Boyanov, M I and Bunker, B A and Yee, N and Environmental Research and Univ. of Notre Dame},
abstractNote = {X-ray absorption fine structure (XAFS) measurements was used at the U L3-edge to directly determine the pH dependence of the cell wall functional groups responsible for the absorption of aqueous UO{sub 2}{sup 2+} to Bacillus subtilis from pH 1.67 to 4.80. Surface complexation modeling can be used to predict metal distributions in water-rock systems, and it has been used to quantify bacterial adsorption of metal cations. However, successful application of these models requires a detailed knowledge not only of the type of bacterial surface site involved in metal adsorption/desorption, but also of the binding geometry. Previous acid-base titrations of B. subtilis cells suggested that three surface functional group types are important on the cell wall; these groups have been postulated to correspond to carboxyl, phosphoryl, and hydroxyl sites. When the U(VI) adsorption to B. subtilis is measured, observed is a significant pH-independent absorption at low pH values (<3.0), ascribed to an interaction between the uranyl cation and a neutrally charged phosphoryl group on the cell wall. The present study provides independent quantitative constraints on the types of sites involved in uranyl binding to B. subtilis from pH 1.67 to 4.80. The XAFS results indicate that at extremely low pH (pH 1.67) UO{sub 2}{sup 2+} binds exclusively to phosphoryl functional groups on the cell wall, with an average distance between the U atom and the P atom of 3.64 {+-} 0.01 Angstroms. This U-P distance indicates an inner-sphere complex with an oxygen atom shared between the UO{sub 2}{sup 2+} and the phosphoryl ligand. The P signal at extremely low pH value is consistent with the UO{sub 2}{sup 2+} binding to a protonated phosphoryl group, as previously ascribed. With increasing pH (3.22 and 4.80), UO{sub 2}{sup 2+} binds increasingly to bacterial surface carboxyl functional groups, with an average distance between the U atom and the C atom of 2.89 {+-} 0.02 Angstroms. This U-C distance indicates an inner-sphere complex with two oxygen atoms shared between the UO{sub 2}{sup 2+} and the carboxyl ligand. The results of this XAFS study confirm the uranyl-bacterial surface speciation model.},
doi = {10.1016/S0016-7037(02)00947-X},
journal = {Geochim. Cosmochim. Acta},
issn = {0016-7037},
number = 22 ; 2002,
volume = 66,
place = {United States},
year = {2002},
month = {1}
}