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Title: Adjustable virtual pore-size filter for automated sample preparation using acoustic radiation force

Abstract

We present a rapid and robust size-based separation method for high throughput microfluidic devices using acoustic radiation force. We developed a finite element modeling tool to predict the two-dimensional acoustic radiation force field perpendicular to the flow direction in microfluidic devices. Here we compare the results from this model with experimental parametric studies including variations of the PZT driving frequencies and voltages as well as various particle sizes and compressidensities. These experimental parametric studies also provide insight into the development of an adjustable 'virtual' pore-size filter as well as optimal operating conditions for various microparticle sizes. We demonstrated the separation of Saccharomyces cerevisiae and MS2 bacteriophage using acoustic focusing. The acoustic radiation force did not affect the MS2 viruses, and their concentration profile remained unchanged. With optimized design of our microfluidic flow system we were able to achieve yields of > 90% for the MS2 with > 80% of the S. cerevisiae being removed in this continuous-flow sample preparation device.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
945697
Report Number(s):
LLNL-JRNL-404158
TRN: US200903%%739
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry, vol. 80, N/A, October 11, 2008, pp. 8447-8452
Additional Journal Information:
Journal Volume: 80
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ACOUSTICS; BACTERIOPHAGES; DESIGN; FOCUSING; PARTICLE SIZE; RADIATIONS; SACCHAROMYCES CEREVISIAE; SAMPLE PREPARATION; SIMULATION; VIRUSES

Citation Formats

Jung, B, Fisher, K, Ness, K, Rose, K, and Mariella, R. Adjustable virtual pore-size filter for automated sample preparation using acoustic radiation force. United States: N. p., 2008. Web.
Jung, B, Fisher, K, Ness, K, Rose, K, & Mariella, R. Adjustable virtual pore-size filter for automated sample preparation using acoustic radiation force. United States.
Jung, B, Fisher, K, Ness, K, Rose, K, and Mariella, R. Thu . "Adjustable virtual pore-size filter for automated sample preparation using acoustic radiation force". United States. https://www.osti.gov/servlets/purl/945697.
@article{osti_945697,
title = {Adjustable virtual pore-size filter for automated sample preparation using acoustic radiation force},
author = {Jung, B and Fisher, K and Ness, K and Rose, K and Mariella, R},
abstractNote = {We present a rapid and robust size-based separation method for high throughput microfluidic devices using acoustic radiation force. We developed a finite element modeling tool to predict the two-dimensional acoustic radiation force field perpendicular to the flow direction in microfluidic devices. Here we compare the results from this model with experimental parametric studies including variations of the PZT driving frequencies and voltages as well as various particle sizes and compressidensities. These experimental parametric studies also provide insight into the development of an adjustable 'virtual' pore-size filter as well as optimal operating conditions for various microparticle sizes. We demonstrated the separation of Saccharomyces cerevisiae and MS2 bacteriophage using acoustic focusing. The acoustic radiation force did not affect the MS2 viruses, and their concentration profile remained unchanged. With optimized design of our microfluidic flow system we were able to achieve yields of > 90% for the MS2 with > 80% of the S. cerevisiae being removed in this continuous-flow sample preparation device.},
doi = {},
url = {https://www.osti.gov/biblio/945697}, journal = {Analytical Chemistry, vol. 80, N/A, October 11, 2008, pp. 8447-8452},
number = ,
volume = 80,
place = {United States},
year = {2008},
month = {5}
}