Final Report for DOE grant no. DE-FG02-04ER63883: Can soil genomics predict the impact of precipitation on nitrous oxide flux from soil
Nitrous oxide is a potent greenhouse gas that is released by microorganisms in soil. However, the production of nitrous oxide in soil is highly variable and difficult to predict. Future climate change may have large impacts on nitrous oxide release through alteration of precipitation patterns. We analyzed DNA extracted from soil in order to uncover relationships between microbial processes, abundance of particular DNA sequences and net nitrous oxide fluxes from soil. Denitrification, a microbial process in which nitrate is used as an electron acceptor, correlated with nitrous oxide flux from soil. The abundance of ammonia oxidizing archaea correlated positively, but weakly, with nitrous oxide production in soil. The abundance of bacterial genes in soil was negatively correlated with gross nitrogen mineralization rates and nitrous oxide release from soil. We suggest that the most important control over nitrous oxide production in soil is the growth and death of microorganisms. When organisms are growing nitrogen is incorporated into their biomass and nitrous oxide flux is low. In contrast, when microorganisms die, due to predation or infection by viruses, inorganic nitrogen is released into the soil resulting in nitrous oxide release. Higher rates of precipitation increase access to microorganisms by predators or viruses through filling large soil pores with water and therefore can lead to large releases of nitrous oxide from soil. We developed a new technique, stable isotope probing with 18O-water, to study growth and mortality of microorganisms in soil.
- Research Organization:
- Northern Arizona Univ., Flagstaff, AZ (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- FG02-04ER63883
- OSTI ID:
- 944079
- Report Number(s):
- DOE/ER/63883; TRN: US201002%%1158
- Country of Publication:
- United States
- Language:
- English
Similar Records
Alterations in soil pH emerge as a key driver of the impact of global change on soil microbial nitrogen cycling: Evidence from a global meta-analysis
Copper availability governs nitrous oxide accumulation in wetland soils and stream sediments
Related Subjects
ABUNDANCE
AMMONIA
BINDING ENERGY
BIOMASS
CLIMATIC CHANGE
DENITRIFICATION
DNA
ELECTRONS
GENES
GREENHOUSE GASES
MICROORGANISMS
MINERALIZATION
MORTALITY
NITRATES
NITROGEN
NITROUS OXIDE
PRECIPITATION
SOILS
STABLE ISOTOPES
VALENCE
VIRUSES
nitrous oxide
genomic analysis
soil ecology
ecosystem research
climate change