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Title: Mouse liver selenium-binding protein decreased in aboundance by peroxisome proliferators.

Abstract

Several studies with two-dimensional gel electrophoresis (2-DE) have shown that the abundance of numerous mouse liver proteins is altered in response to treatment with chemicals known to cause peroxisome proliferation. The peptide masses from tryptic digests of two liver proteins showing dramatic decreases in abundance in response to numerous peroxisome proliferators were used to search sequence databases. The selenium-binding protein 2 (SBP2 formerly 56 kDa acetaminophen-binding protein, AP 56) and selenium-binding protein 1 (SBP1 formerly 56 kDa selenium-binding protein, SP 56) in mouse liver, proteins with a high degree of sequence similarity, were the highest ranked identities obtained. Identity with SBP2 was subsequently confirmed by immunodetection with specific antiserum. Treatment of mice with 0.025% ciprofibrate resulted in the more basic of this pair of proteins being decreased to 30% of control abundance while the acidic protein was decreased to 7% of the control amount. Dexamethasone treatment, in contrast, caused increases of 80% and 20% in the abundance of the acidic and basic forms, respectively. Administration of dexamethasone to mice in combination with ciprofibrate produced expression of the acidic SBP2 at 23% of the control level and the basic SBP2 at 36%, a slightly moderated reduction compared with the decrease thatmore » occurred with ciprofibrate alone. These data suggest that peroxisome proliferators such as ciprofibrate cause a decrease in the abundance of the SBP2, which leads to increased cell proliferation, even in the presence of an inhibitor such as dexamethasone. Such a decrease in SBP, thought to serve as cell growth regulation factors, could be central to the nongenotoxic carcinogenicity of the peroxisome proliferators observed in rodents.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
942950
Report Number(s):
ANL/BIO/JA-36166
Journal ID: ISSN 0173-0835; TRN: US201002%%456
DOE Contract Number:  
DE-AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Electrophoresis
Additional Journal Information:
Journal Volume: 21; Journal Issue: 11 ; Jun. 2000; Journal ID: ISSN 0173-0835
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CELL PROLIFERATION; DEXAMETHASONE; ELECTROPHORESIS; GELS; GROWTH; IMMUNE SERUMS; LIVER; MICE; PEPTIDES; PROTEINS; REDUCTION; RECEPTORS; RODENTS; SELENIUM

Citation Formats

Giometti, C. S., Liang, X., Tollaksen, S. L., Wall, D. B., Lubman, D. M., Subbarao, V., and Sambasiva Rao, M. Mouse liver selenium-binding protein decreased in aboundance by peroxisome proliferators.. United States: N. p., 2000. Web. doi:10.1002/1522-2683(20000601)21:11<2162::AID-ELPS2162>3.3.CO;2-J.
Giometti, C. S., Liang, X., Tollaksen, S. L., Wall, D. B., Lubman, D. M., Subbarao, V., & Sambasiva Rao, M. Mouse liver selenium-binding protein decreased in aboundance by peroxisome proliferators.. United States. doi:10.1002/1522-2683(20000601)21:11<2162::AID-ELPS2162>3.3.CO;2-J.
Giometti, C. S., Liang, X., Tollaksen, S. L., Wall, D. B., Lubman, D. M., Subbarao, V., and Sambasiva Rao, M. Thu . "Mouse liver selenium-binding protein decreased in aboundance by peroxisome proliferators.". United States. doi:10.1002/1522-2683(20000601)21:11<2162::AID-ELPS2162>3.3.CO;2-J.
@article{osti_942950,
title = {Mouse liver selenium-binding protein decreased in aboundance by peroxisome proliferators.},
author = {Giometti, C. S. and Liang, X. and Tollaksen, S. L. and Wall, D. B. and Lubman, D. M. and Subbarao, V. and Sambasiva Rao, M.},
abstractNote = {Several studies with two-dimensional gel electrophoresis (2-DE) have shown that the abundance of numerous mouse liver proteins is altered in response to treatment with chemicals known to cause peroxisome proliferation. The peptide masses from tryptic digests of two liver proteins showing dramatic decreases in abundance in response to numerous peroxisome proliferators were used to search sequence databases. The selenium-binding protein 2 (SBP2 formerly 56 kDa acetaminophen-binding protein, AP 56) and selenium-binding protein 1 (SBP1 formerly 56 kDa selenium-binding protein, SP 56) in mouse liver, proteins with a high degree of sequence similarity, were the highest ranked identities obtained. Identity with SBP2 was subsequently confirmed by immunodetection with specific antiserum. Treatment of mice with 0.025% ciprofibrate resulted in the more basic of this pair of proteins being decreased to 30% of control abundance while the acidic protein was decreased to 7% of the control amount. Dexamethasone treatment, in contrast, caused increases of 80% and 20% in the abundance of the acidic and basic forms, respectively. Administration of dexamethasone to mice in combination with ciprofibrate produced expression of the acidic SBP2 at 23% of the control level and the basic SBP2 at 36%, a slightly moderated reduction compared with the decrease that occurred with ciprofibrate alone. These data suggest that peroxisome proliferators such as ciprofibrate cause a decrease in the abundance of the SBP2, which leads to increased cell proliferation, even in the presence of an inhibitor such as dexamethasone. Such a decrease in SBP, thought to serve as cell growth regulation factors, could be central to the nongenotoxic carcinogenicity of the peroxisome proliferators observed in rodents.},
doi = {10.1002/1522-2683(20000601)21:11<2162::AID-ELPS2162>3.3.CO;2-J},
journal = {Electrophoresis},
issn = {0173-0835},
number = 11 ; Jun. 2000,
volume = 21,
place = {United States},
year = {2000},
month = {6}
}