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Title: Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms

Abstract

The photobiological production of H{sub 2} gas, using water as the only electron donor, is a property of two types of photosynthetic microorganisms: green algae and cyanobacteria. In these organisms, photosynthetic water splitting is functionally linked to H{sub 2} production by the activity of hydrogenase enzymes. Interestingly, each of these organisms contains only one of two major types of hydrogenases, [FeFe] or [NiFe] enzymes, which are phylogenetically distinct but perform the same catalytic reaction, suggesting convergent evolution. This idea is supported by the observation that each of the two classes of hydrogenases has a different metallo-cluster, is encoded by entirely different sets of genes (apparently under the control of different promoter elements), and exhibits different maturation pathways. The genetics, biosynthesis, structure, function, and O{sub 2} sensitivity of these enzymes have been the focus of extensive research in recent years. Some of this effort is clearly driven by the potential for using these enzymes in future biological or biohybrid systems to produce renewable fuel or in fuel cell applications.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
941437
DOE Contract Number:
AC36-99-GO10337
Resource Type:
Journal Article
Resource Relation:
Journal Name: Annual Review of Plant Biology; Journal Volume: 58; Journal Issue: 2007
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN; 59 BASIC BIOLOGICAL SCIENCES; ALGAE; BINDING ENERGY; BIOSYNTHESIS; CYANOBACTERIA; ELECTRONS; ENZYMES; FUEL CELLS; GENES; GENETICS; HYDROGEN; HYDROGENASES; MICROORGANISMS; PHOTOPRODUCTION; PRODUCTION; PROMOTERS; SENSITIVITY; VALENCE; WATER; Hydrogen

Citation Formats

Ghirardi, M. L., Posewitz, M. C., Maness, P. C., Dubini, A., Yu, J., and Seibert, M. Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms. United States: N. p., 2007. Web. doi:10.1146/annurev.arplant.58.032806.103848.
Ghirardi, M. L., Posewitz, M. C., Maness, P. C., Dubini, A., Yu, J., & Seibert, M. Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms. United States. doi:10.1146/annurev.arplant.58.032806.103848.
Ghirardi, M. L., Posewitz, M. C., Maness, P. C., Dubini, A., Yu, J., and Seibert, M. Mon . "Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms". United States. doi:10.1146/annurev.arplant.58.032806.103848.
@article{osti_941437,
title = {Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms},
author = {Ghirardi, M. L. and Posewitz, M. C. and Maness, P. C. and Dubini, A. and Yu, J. and Seibert, M.},
abstractNote = {The photobiological production of H{sub 2} gas, using water as the only electron donor, is a property of two types of photosynthetic microorganisms: green algae and cyanobacteria. In these organisms, photosynthetic water splitting is functionally linked to H{sub 2} production by the activity of hydrogenase enzymes. Interestingly, each of these organisms contains only one of two major types of hydrogenases, [FeFe] or [NiFe] enzymes, which are phylogenetically distinct but perform the same catalytic reaction, suggesting convergent evolution. This idea is supported by the observation that each of the two classes of hydrogenases has a different metallo-cluster, is encoded by entirely different sets of genes (apparently under the control of different promoter elements), and exhibits different maturation pathways. The genetics, biosynthesis, structure, function, and O{sub 2} sensitivity of these enzymes have been the focus of extensive research in recent years. Some of this effort is clearly driven by the potential for using these enzymes in future biological or biohybrid systems to produce renewable fuel or in fuel cell applications.},
doi = {10.1146/annurev.arplant.58.032806.103848},
journal = {Annual Review of Plant Biology},
number = 2007,
volume = 58,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}