Circular permutant GFP insertion folding reporters
- Santa Fe, NM
- Los Alamos, NM
Provided are methods of assaying and improving protein folding using circular permutants of fluorescent proteins, including circular permutants of GFP variants and combinations thereof. The invention further provides various nucleic acid molecules and vectors incorporating such nucleic acid molecules, comprising polynucleotides encoding fluorescent protein circular permutants derived from superfolder GFP, which polynucleotides include an internal cloning site into which a heterologous polynucleotide may be inserted in-frame with the circular permutant coding sequence, and which when expressed are capable of reporting on the degree to which a polypeptide encoded by such an inserted heterologous polynucleotide is correctly folded by correlation with the degree of fluorescence exhibited.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM
- Sponsoring Organization:
- United States Department of Energy
- DOE Contract Number:
- W-7405-ENG-36; FG02-98ER62647
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Number(s):
- 7,390,640
- Application Number:
- 11/360,178
- OSTI ID:
- 941141
- Country of Publication:
- United States
- Language:
- English
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Circular permutant GFP insertion folding reporters
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