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Title: Methods for increasing the production of ethanol from microbial fermentation

Abstract

A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

Inventors:
 [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. Fayetteville, AR
  2. Bethlehem, PA
Publication Date:
Research Org.:
BIOENGINEERING RESOURCES, INC.
Sponsoring Org.:
USDOE
OSTI Identifier:
941118
Patent Number(s):
7,285,402
Application Number:
10/311,655
Assignee:
Emmaus Foundation, Inc. (Fayetteville, AK)
DOE Contract Number:  
FC04-94AL98770
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Gaddy, James L, Arora, Dinesh K, Ko, Ching-Whan, Phillips, John Randall, Basu, Rahul, Wikstrom, Carl V, and Clausen, Edgar C. Methods for increasing the production of ethanol from microbial fermentation. United States: N. p., 2007. Web.
Gaddy, James L, Arora, Dinesh K, Ko, Ching-Whan, Phillips, John Randall, Basu, Rahul, Wikstrom, Carl V, & Clausen, Edgar C. Methods for increasing the production of ethanol from microbial fermentation. United States.
Gaddy, James L, Arora, Dinesh K, Ko, Ching-Whan, Phillips, John Randall, Basu, Rahul, Wikstrom, Carl V, and Clausen, Edgar C. 2007. "Methods for increasing the production of ethanol from microbial fermentation". United States. https://www.osti.gov/servlets/purl/941118.
@article{osti_941118,
title = {Methods for increasing the production of ethanol from microbial fermentation},
author = {Gaddy, James L and Arora, Dinesh K and Ko, Ching-Whan and Phillips, John Randall and Basu, Rahul and Wikstrom, Carl V and Clausen, Edgar C},
abstractNote = {A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.},
doi = {},
url = {https://www.osti.gov/biblio/941118}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 23 00:00:00 EDT 2007},
month = {Tue Oct 23 00:00:00 EDT 2007}
}

Works referenced in this record:

Introduction to the Proceedings of the Fourteenth Symposium on Biotechnology for Fuels and Chemicals
journal, September 1993


Biological conversion of coal and coal-derived synthesis gas
journal, December 1993